Peptides representing epitopic sites for bacterial and viral meningitis causing agents and their CNS carrier and uses thereof

ABSTRACT

Peptides comprising a Meningitis Related Homologous Antigenic Sequence (MRHAS) are provided. The MRHAS is found in meningitis-causing organisms and chemokines involved in cell chemotaxis. The peptides are useful as antigens and vaccines for detection, diagnosis and treatment of meningitis.

This application is a divisional of application Ser. No. 08/127,499, filed Sep. 28, 1993 allowed patent application.

FIELD OF THE INVENTION

This invention relates to the application of immunological techniques that provide novel materials useful in the diagnosis, treatment and vaccination against meningitis caused by either bacterial or viral agents. These techniques include the production and application of novel monoclonal antibodies, peptides, and mixtures and combinations thereof that are useful for detecting meningitis infections. The techniques also include eliciting antibodies specific to meningitis causing agents. These immunological techniques may also be applied to the treatment of such disease.

BACKGROUND OF THE INVENTION

The term meningitis is a general one, referring to the inflammatory response to infection of the meninges and the cerebrospinal fluid (CSF). See Roos, "Chapter 16", in Scheld, et al. eds., 1991, Infections of the Central Nervous System:335-403 which is incorporated herein in its entirety by reference.

The fact that the inflammatory response occurs in the proximity of the brain and in the space limited by a rigid cranium, makes these infections serious and life threatening. Most patients exhibit nonspecific clinical signs and symptoms such as fever, irritability, altered mental status usually accompanied by vomiting and loss of appetite. In children one year of age and older, photophobia and headache are common complaints. Specific clinical signs indicative of meningitis are neck rigidity and pain on neck flexicn. Brudzinski's sign (neck flexion producing knee and hip flexicn) and Kernig's sign (difficulty and pain in raising extended leg) are other useful clinical signs.

In infants less than 6 months old, early diagnosis of meningitis is difficult because signs of meningitis are not prominent and neck rigidity is often absent. Such patients commonly exhibit fever, respiratory distress, other signs of sepsis, and convulsions. Bulging anterior fontanelle due to increased intracranial pressure may be the only specific sign.

Petechiae (or rash) is most commonly present in meningococcal infections. In severe meningococcal infections with bacteraemia, petechiae and shock may develop with alarming rapidity. Convulsions at some point in the illness occur in about 30% of the cases. This number is often higher in neonares and infants under one year of age. Other acute complications include septic shock, disseminated intravascular coagulation, syndrome of inappropriate antidiuretic hormone, increased intracranial pressure, and diabetes insipidus. Convulsions and coma appearing with 24 hours accompanied by high fever indicates serious infection (Stutman & Marks, 1987, Clin. Ped. 26:432-438).

A diverse array of both bacteria and viruses cause meningitis, the infectivity of which is dependent on a complex array of factors, including virulence of the organisms, the carrier state, and the host's humoral immune response.

Viruses generally cause milder forms of meningitis (eg. meningomyelitis and aseptic meningitis) with a short clinical course and reduced mortality. Agents most commonly associated are coxsackievirus A (types 2,4,7,9,10), B (types 1-6), polio virus, echoviruses (types 1-34, except 12,24,26,29,32-34), enteroviruses (types 70, 71), human immunodeficiency virus-1 (HIV-1), and rubella virus (RV). See Melnick, "Chapter 33" and Cooper, "Chapter 42" in Fields, et al., eds., 1985, Virology: 739-794 and 1005-1032, respectively; and Rotbart, "Chapter 3", in Scheld et al., 1991, infra:19-33 which are all incorporated herein by reference.

Rubella is possibly the most common cause of viral meningitis. Moreover, the most common chemical sequelae of rubella infection of young children are meningitis, meningomyelitis and rubella associated panencephalitis. Rubella is a highly contagious disease, usually associated with childhood, and is characterized by a general rash and a mild fever. Sub-clinical infections are also common. Its clinical aspects have been confused with measles, which it closely resembles. Since its early discovery in Germany, Rubella is often referred to as German measles. The infection of a pregnant woman poses the greatest risk when infection of the fetus can lead to spontaneous abortion or an array of abnormalities called the Congenital Rubella Syndrome in the newborn. Damage most frequently involves cardiac abnormalities, deafness, cataracts, blindness and Central Nervous System (CNS) disorders including microencephaly.

The rubella virion is a spherical, enveloped virus, approximately 60 nm in diameter, and is a member of the Togaviridae. It's genome is a 10 Kb plus single-stranded RNA. The outer envelope is comprised of lipoproteins derived from the infected host cell, and it appears to have two viral encoded glycoproteins, E1 (58 Kd) and E2 (42-47 Kd), responsible for the hemagglutination activity of the virus. Its core protein is a non-glycosylated nucleocapsid protein with an approximate weight of 33 Kd. It appears that the core, E1, and E2 are all derived from the same parent protein--Structural Polyprotein. See Clark et al., 1987, Nucl. Acids Res. 15:3041-3057; Dominguez, et al., 1990, Virolcgy 177:225-238, both which are incorporated herein by reference. Three strains of wild type RV (M33, Therien, Judith) and a vaccine strain (HPV77) of RV have been identified and sequenced (Zheng et alo, 1988, Arch. Virol. 98:189-197 incorporated herein in its entirety by reference). Between these different wild types strains, there exists minor variations in the amino acid sequence of the Structural Polyprotein (Dominguez, infra; Clarke, infra).

The detection of RV in diagnosis has in the past proven difficult, largely because the virus grows to low titers in the tissue cultures and is highly liable, making it technically difficult to isolate and purify (Ho-Terry et al., 1986, Arch. Virol. 87: 219-228).

The detection of RV in the CNS presents additional technical problems. It has been known since 1941 that the RV can infect cells of the CNS (Gregg, 1941, Trans. Ophthalmol. Soc. Aust. 3:35-46). However, it has proven difficult to reliably demonstrate the presence of the RV in infected brain tissue. Persistent infection of the CNS has been well documented in the congenital rubella syndrome (Desmond et al., 1967, J. Pediat., 7:311-331), and in the neuropathology if progressive rubella panencephalitis of late onset occurs where the virus has been isolated from brain biopsy material (Townsend et al., 1975, N. Engl. J. Med. 292:990-993; Cremer et al., 1979, J. Gen. Virol. 29:143-153). Less commonly documented are the wide range of neuropathies known to follow exposure to the RV. These include encephalitis, meningomyelitis, and bilateral optic neuritis (Connolly et al., 1975, Brain 98:583-594). Moreover, the report of a diffuse myelitis following RV in cells of the nervous system requires further investigation (Holt et al., 1975, Brit. Med. J., 7:1037-1038).

RV-directed polypeptide synthesis in normal rat glial cells in continuous tissue culture has been studied (Singh & Van Alstyne, 1978, Brain Res. 155:418-421). Unlike a productive rubella virus infection in permissive murine L (muscle) cells, infection of normal glial cells resulted in no detectable progeny virons in tissue culture supernatants and no detectable rubella 33 Kd core protein in infected cell lysates (Pope and Van Alstyne, 1981, Virology 124:173-180). Furthermore, exposure of infected gila to dibutyryl cyclic adenine monophosphate reversed the restriction, resulting in the appearance of the 33 Kd rubella nucleocapsid protein in infected cell lysates and the appearance of mature progeny virions in tissue culture supernatants (Van Alstyne and Paty, 1983, Virology 124:173-180).

Others have reported a lack of synthesis of the structural M protein in measles virus-infected brain cells obtained from subacute sclerosing panencephalitis autopsy material established in tissue culture (Hall and Choppin, 1979, Virology 99:443-447). Also, it is known that the incomplete synthesis of some Herpes specific structural proteins occurs during a nonpermissive infection of some cells of nervous system origin (Adler et al., 1978, J. Gen. Virology 39:9-20).

Taken together, these data indicate that even very different viruses may undergo restricted replication in brain cells. The synthesis of a limited number of viral gene products could account for incomplete virion assembly, the translation of polypeptides of variable molecular weights, alterations in the immune response to input virus, and difficulties in successful virus isolation from infected brain tissue.

Therefore, there remains a need for a diagnostic system which would detect RV protein antigens in CNS tissue in both the presence as well as the absence of an active, productive infection.

Early diagnostic tests were based on the hemagglutinating properties of its external glycoproteins. Commonly, the hemagglutination inhibition assays relied on the presence of antibodies to the RV hemagglutinin (HA) in the serum samples to inhibit the vital-mediated hemagglutination of chick red blood cells (Herrmann, "Rubella Virus", 1979, in Diagnostic Procedures For Vital, Rickettsial And Chlamydial Infections:725-766). The presence of high inhibition, indicated the indirect measurement of antibodies to the HA protein, and thereby, a recent rubella infection.

More recent tests employ enzyme-labelled antibodies in the enzyme-linked-immunosorbent assays (ELISA) (Voller & Bidwell, 1975, Br. J. Exp. Pathol. 56:338-339 incorporated herein by reference). These assays are also indirect tests to measure the amount of circulating antibody to RV as an indication of infection. Indirect ELISA tests for RV employ bound viral antigens on a plastic microwells and the presence of bound antibodies linked to enzymes such as horseradish peroxidase.

There are several problems with the use of the indirect RV ELISA kits. These relate to low antibody titers observed with RV infection, the need for elaborate "cut-off" value calculations to eliminate background binding, the limited use of the test in the detection of low levels of specific viral antigens present in chronic CNS infection, and the tedious and time consuming nature of the test performance.

A different use of monoclonal antibodies and their corresponding synthetic peptide epitopes may prove more useful in detecting RV infection in the CNS. There has been discussion that refers to the use of three non-competing monoclonal antibodies directed against the E1 glycoprotein, but this system has not been applied to CNS-specific diagnostics (Terry et al., 1988, Arch. Virol. 98, 189-197 incorporated herein by reference).

Therefore, there is clearly a need for a rapid and a sensitive diagnostic test for the detection of the RV in CNS infection.

Furthermore, a live, attenuated rubella vaccine has been developed (Parkman et al., 1966, N. Engl. J. Med 275: 569-574). This vaccine is immunogenic in at least 95% of the recipients, and does confer protection against reinfection, in spite of the fact that it induces antibody levels which are significantly lower than those generated by wild type virus infection. However, a serious drawback associated with the administration of the attenuated vaccine is the significant proportion of adult females that go on to develop rubella-associated arthritis. Furthermore, recently immunized individuals still harbour infectious virus and are therefore infectious, proving dangerous to pregnant women with whom they may be in contact.

Therefore, there is also a need for a non-infectious, innocuous vaccine. Such a vaccine could possibly be constructed from synthetic or recombinant peptides of RV proteins. Moreover, no epitope has yet been identified which would induce only neutralizing antibodies, necessary for conferring effective vaccine protection.

Another virus responsible for meningitis is the Human Immunodeficency Virus-1 (HIV-1). HIV-1 is a human retrovirus which has been identified as the etiological agent of AIDS, an infectious and fatal disease transmitted through intimate sexual contact and exposure to contaminated blood or blood products. HIV-1 is related to the lentiviruses on the basis of its biological and in vitro characteristics, morphology and nucleotide sequences. It is also referred to as Human T-cell Lymphotrophic Virus type III, Lymphadenopathy Associated Virus, and AIDS Associated Retrovirus (Gallo, et al., 1984, Science, 224:500-503; Sarngadharan, et al., 1984, Science, 224:506-508; Barre-Sinoussi, et al., 1983, Science, 220:868-871; Levy, 1984, Science, 225:840-842; Gonda et al., 1985 Science, 227:177-179; Stephan, et al., 1986, Science, 231:589-594). Much interest has been focused on the effect of the long term, persistent infection of the immune system, by HIV-1. Recent information indicates that the virus moves from blood to the lymph nodes and thymus where it remains active, culminating in viremia, a precipitous drop in the CD4+T-cell count, and one or more of the several symptoms known as AIDS.

However, primary HIV-1 infection itself results in an immediate set of defined clinical features. Commonly, an acute febrile illness resembling influenza or mononucleosis is noted. In addition, lymphocytic meningitis may accompany the febrile illness and the patient may then be presented with headache, stiff neck and photophobia, as well as rigors, arthralgias and myalgias, truncal maculcpapular rash, urticaria, abdominal cramps and diarrhea (Ho, 1985, Ann. Internal Medicine 103:880-883).

While some patients remain asymptomatic for up to 3 months preceding their seroconversion, indicating that HIV-1 infection may be subclinical, primary infection should be included in the differential diagnosis of prolonged febrile illnesses in persons at risk for AIDS. The presence of a maculopapular or urticarial rash, or lymphocytic meningitis is compatible with this diagnosis. Hence, early recognition of the varied syndromes associated with this virus might permit effective treatment before immunologic abnormalities become established.

There is, therefore, the need for a rapid, direct diagnostic test for viral meningitis, prior to seroconversion, when the transient meningitis may represent the initiation of a more serious, long term HIV-1 related illness.

Currently, one of the most commonly used direct tests for HIV-1 infection employs the following approaches: (i) direct culturing of virus from infected blood or,blood cells and subsequent in vitro propagation of the virus in lymphocyte cultures; (ii) measuring reverse transcriptase levels; (iii) immunocytochemical staining of viral proteins; (iv) electron microscopy; (v) hybridization of nucleic acid probes; and measuring HIV-1 antigens with enzyme immunoassays (Goudsmit et al., 1986, Brit. Med. J., 2993:1459-1462; Caruso et al., 1987, J. Virol. Methods, 17:199-210).

The HIV-1 appears to have at least three core protein (p17, p24, and p15) that are derived from a core polyprotein called gag polyprotein. See Muesing, et al., 1985, Nature 313:450-458 incorporated herein by reference. The gag polyprotein in the LV isolate of HIV-1 is 478 amino acids long and the three mature core proteins appear to be derived as p17 from amino acid sequence numbers 1-132, p24 from amino acid sequence numbers 133-391, and p15 from amino acid sequence numbers 392-478 (Muesing, infra). Moreover, it appears that the HIV-1 (LAV-la isolate) also has at least one capsid transmembrane glycoprotein derived from a 861 amino acid long Envelope Polyprotein (Wain-Hobson, et al., 1985, Cell 40:9-17 incorporated herein by reference).

The enzyme immunoassays have clearly shown the diagnostic importance of the presence of the p24 core protein. A correlation has been established between viremia, the decline of antibodies to p24, and the progression of symptoms from the asymptomatic seropositivity to fully expressed AIDS (Lange et al., 1986, Brit. Med. J., 293:1459-1462; Paul et al., 1987, J. Med. Virol., 22:357-363; Forster et al., 1987, AIDS, 1:235-240). A decline in the p24 level has also been observed to occur in patients treated with AZT (Chaisson et al., 1986, New Eng. J. Med., 315:1610-1611).

Assays for the direct detection of p24 are currently on the market (Allain, infra; Forster, infra). These assays use the same sandwich format in which serum samples are incubated with bound and enzyme-labelled anti-p24-antibodies to form an antibody/p24-antigen-antibody sandwich. Antigen levels of approximately 50 picograms/ml can be detected, when the antigen concentration is read from a standard curve constructed with a set of p24 standards of known concentrations. The tests are tedious and time consuming to perform, require dilutions of patients' sera, and do not provide information regarding the comparisons of rising antigen and concomitant declining antibody levels necessary to evaluate laboratory findings.

Therefore, the need to rapidly and effective diagnostic test to screen large numbers of a symptomatic individuals for the presence of HIV-1 virus in individuals is clear.

There is also an urgent need for a vaccine to afford protection against transmission of AIDS by individuals who are not detected by current diagnostic tests.

However, there are significant difficulties inherent in designing a vaccine which will confer protection against HIV-1. The vaccine must differentiate between HIV-1 and the closely-related virus, HIV-2. The rapid rate of HIV-1 mutation requires that the antigen(s) be highly conserved. Moreover, the HIV-1 infection of a small subset of T cells requires the killing of an integral part of the immune cell network, with unknown consequences, to completely eradicate the virus. In addition, vaccinated antigens could enter lymph nodes and stimulate B cells to produce cytokines that in turn stimulate HIV-1 infection of T cells, and thereby having a reverse effect, causing a more rapid onset of AIDS.

Peptides from gp120, gp160, gp41, gp120 +gp41, p17 and p14 are currently being employed for vaccine production by several companies and universities (Spalding, 1992, Biotech. 10:24-29.) However, these peptides are being tested for their ability to solely induce B cells to produce neutralizing antibody.

Therefore, there is an urgent need for the selection of HIV-1 peptides which would serve as appropriate B cell stimulators, to produce protective, neutralizing antibody, as well as appropriate cytokine blockers to prevent HIV-1 infection of T-cells. To date, no known combination of such peptides has been shown to protect against AIDS infection.

Bacteria are the other major cause of meningitis. Approximately 70% of all cases of bacterial meningitis occur in children under the age of 5 years; three bacterial species cause 84% of all meningitis cases reported in the United States: Haemophilus influenza type B, and Streptococcus pneumoniae and Neisseria meningitidis (Roos, infra; Stutman, infra). Less prevalent bacterial species include Pseudomonas aerugensosa, Staphylococci, Mycobacteria and Listeria species.

All strains of Haemophilus influenzae (H. influenza) are divided into two groups: typeable strains which commonly have a capsule, and nontypeable strains which do not. Typing of the encapsulated strains is accomplished by serological techniques, using reference antisera. Types a to f have been identified in this way. Those strains which fail to react with any of the reference antisera are classified as nontypeable.

The most frequent cause of neonatal meningitis and other invasive infections in the United States is the encapsulated H. influenzae type b (Hib) (Fraser et al., 1974, Am. J. Epidemiol,. 100:29-34). While the major incidence of childhood meningitis occurs between the ages of one and five years, 60% of the meningitis cases due to Hib occur in children under the age of two years.

The nontypeable H. influenzae are known to cause meningitis, pneumonia, bacteraemia, postpartum sepsis, and acute febrile tracheobronchitis in adults (Murphy et al., 1985, J. Infect. Diseases, 152:1300-1307). About 20 to 40% of all cases of otitis media are caused by this H. influenzae, which is a frequent etiologic agent of otitis media in children and young adults. Since infection confers no long lasting immunity, repeated infections of the same organism is frequently observed. These chronic ototis media infections are treated by administration of antibiotics, and drainage of the inner ear, where such a procedure is deemed necessary. H. influenzae strains have also been implicated as a primary cause of sinusitis (Cherry & Dudley, 1981, in Feigin & Cherry eds., Textbook of Pediatric Infectious Diseases:103-105). Nontypeable H. influenzae are also known to cause neonatal sepsis.

A vaccine is currently available for protection against typeable influenzae, and employs the capsular polysaccharide antigen of Hib, polyribosyl ribitol phosphate (Smith et al., 1973, Pediatrics, 52:637-644; Anderson et al., 1972, J. Clin. Inv., 51:31-88). However, Anti-PRP antibody is not effective in conferring protection against non-typeable H. influenzae infection. Thus, all available vaccines against H. influenzae are all directed against Hib, and all elicit anti-PRP antibody to confer protection. Since the non-typeable H. influenzae lack the PRP capsule, no vaccine is efficacious against this group.

However, there does appears that H. Influenzae exhibits an outer membrane lipoprotein referred to as p4 (Green, et al., 1992, EMBL Bank, incorporated herein by reference). The p4 protein appears to be derived from the Lipoprotein E Precursor, the precursor protein being 274 amino acids in length. (SEQ ID NO: 17)

There is therefore a clear need for both a method of diagnosis for this disease as well as a vaccine which would protect against both typeable as well as nontypeable H. influenzae. It is possible that the p4 lipoprotein providing a source for such a vaccine.

Streptococcus pneumoniae is the leading cause of community-acquired bacterial pneumonia (pneumococcal diseases), with approximately 500,000 cases a year reported in the United States. Bacterial pneumonia is most prevalent among the very young, the elderly and immuno-compromised persons. In infants and children, pneumococci are the most common bacterial cause of pneumonia, otitis media and bacteraemia and a less common cause of meningitis (causing 20-25% of reported cases).

Pneumococci are carried in the respiratory tract of a significant number of healthy individuals. But, in spite of the high carriage rate, its presence does not necessarily imply infection. However, if one of the highly pathogenic pneumococcal types, such as S. pneumoniae, is isolated from rusty-colored sputum (also containing a large number of polymorphonuclear leucocytes), body fluids, blood cultures, or specimens collected via transtracheal or lung puncture from the lower respiratory tract, its detection is usually significant.

S. pneumoniae is a gram positive bacteria. Proteins located on the cell surface of many gram positive bacteria are frequently involved in virulence and host immunity and have, in the past, been used in typing these bacteria and in immunoprotection studies. There are a large number of S. pneumoniae strains, classified into serotypes based on their surface carbohydrate structures. There are also many cell surface proteins associated with S. pneumoniae. Surface proteins that exhibit antigenic variation (by antigenic shirt or drift) make the identification of a common but exclusive cell surface antigen difficult and may provide the organism with an additional mechanism for evading the host immune response.

Detection of this bacteria at an early stage is essential to facilitate treatment of the infection. Thus, it is important to be able to quickly identify whether S. pneumoniae is present in a patient and to be able to follow the effect of antibiotic treatment on the bacteria. As available immunoassays for S. pneumoniae antigen detection are deficient for lack of specificity and/or sensitivity, there remains the need for an improved method of such detection.

Monoclonal antibody (Mab) technology has recently provided researchers with tools to reproducibly and accurately analyze the cell surface components of S. pneumoniae. Hence S. pneumoniae proteins are of interest to epidemiologists as they may provide a method of detection as well as for vaccines against the bacteria.

One such cell surface protein is Streptococcus pneumoniae pneumonococcal surface protein A (pspA) (Yother, 1992, J. Bacteriol. 174:601-609 incorporated herein by reference). The complete sequence of this protein is known.

It is known that one such pneumonococcal vaccine has been developed which incorporates the capsular polysaccharide antigens of 23 prevalent serotypes of pneumococci. These serotypes are responsible for 87% of pneumococcal disease in the United States. This second generation vaccine replaced a 14-valent polysaccharide vaccine available since 1977. However, the U.S. Department of Health and Human Services has stated that a more immunogenic pneumococcal vaccine is needed, particularly for children younger than 2 years of age. This necessity exists because the 23-valent vaccine is poorly immunogenic in this age group. Consequently, the use of the vaccine is not recommended in children with recurrent upper respiratory diseases, such as otitis media and sinusitis. Furthermore, the 23-valent vaccine is only 44-61% efficacious when administered to persons over 65 years old, and revaccination is not advised. Thus, there remains a clear need for an improved pneumococcal vaccine.

Neisseria meningitis (N. Meningitis) is one of the leading causes of community-acquired bacterial meningitis, causing 10.3% of cases in the United States between 1978-1981 (Tunkel et al., 1990 Annals of Internal Medicine, 112: 610-623). Meningococcal meningitis is most prevalent among infants between 6-12 months and adolescents (Larter & Master, 1992, Am. J. Med.--Infectious Disease Symposium:120-123). In addition to meningococemia, other less commonly associated diseases such as conjunctivitis, sinusitis, endocarditis, and primary pneumonia can occur (Duerden, 1988, J. Med. Microbiol., 26:161-187).

N. meningitidis bacterium are carried in the nasopharynx of 10-15% of healthy individuals. In spite of the high carriage rate, its presence does not necessarily imply infection. However, isolation of N. meningitidis from cerebral spinal fluid or blood culture is significant (Stutman, infra; Mendelson & Dascal, 1992, Can. J. of Diag., 9:47-57; Martin, 1983, Am. J. Med.:120-123).

N. meningitidis is a gram negative bacteria. Proteins located on the cell surface of many gram negative bacteria have, in the past, been used in typing and immunoprotective studies. There are a large number of N. meningtidis strains and there are many cell surface proteins associated with N. meningtidis. This has made identification of a common but exclusive cell surface antigen difficult.

Detection of this bacteria at an early stage is essential to facilitate treatment of the infection (Stutman, infra). Thus, it is important to possess the ability to identify whether N. meningtidis is present in a patient and to follow the effect of antibiotic treatment on the bacteria. As available immunoassays for N. meningtidis antigen detection have shown lack of specificity and/or sensitivity, there remains the need for an improved method of such detection.

As Mab technology has recently provided researchers with tools to accurately analyze the cell surface components of this bacteria, N. meningtidis proteins are of interest to the epidemiologists as they may provide for a new method of detection as well as a vaccines against it.

One such cell surface protein is the Opacity-Related Protein POPM3 (Stern, 1987, Mol. Microbiol. 1:5-12 incorporated herein by reference). The complete sequence of this 170 amino acid protein is known.

Most meningococcal vaccines have been developed using capsular polysaccharides. One particularly quadravalent vaccine incorporates polyssacharide antigens of serogroups A, C, W and Y, meningococci. However, these serogroups are responsible for less than 49% of memingococcal disease in the United States. No capsular polyssacharide vaccine is available for serogroup B N. meningtidis, which is the most prevalent serogroup, since it is poorly immunogenic. Moreover, polyssacharide vaccines are poorly immunogenic in infants because they are T lymphocyte independent antigens which are inefficient at inducing an immunologic memory. Furthermore, no cross protection between serogroups occurs. Thus, there remains the need for an improved meningococcal vaccine.

It follow then, that there remains a need for at least two products relating to N. meningtidis. The first being a rapid, specific, and sensitive diagnostic test for all strains of N. meningtidis, that does not give false positive results. What is optimally desired is a Mab that will recognize a cell surface antigen that is universally present in most, if not all, strains of N. meningitidis, and, at the same time does not recognize other non-meningitidis causing organisms or material which may be found in conjunction with N. meningtidis. Secondly, it is desirous that the Mab and said protein be used in research towards development of an improved vaccine.

In addition the three major causes of bacterial meningitis, there are other bacterial agents responsible for the disease. One such agent is L. monocytogenes, a motile, grampositive, rod-shaped microorganism belonging to the genus Listeria. This genus is widely distributed in nature-found in soil, water, vegetation and many animal species. See Bille & Doyle, 1990, "Listeria and Erysipelothrix" in Burbert, et al., Manual of Clinical Microbiology 5th ed.:231 which is incorporated herein by reference. Two Listeria species, L. murrayi and L. grayi, are rarely isolated and are presently considered nonpathogenic. However, five other species are genomically related and include three hemolytic species (L. monocytogenes, L. seeligeri and L. ivanovii) and two nonhemolytic species (L. innocua, and L. welshimeri). Of these, only L. monocytogenes, and sometimes L. ivanovii are human pathogens. L. ivanovii is mostly pathogenic for animals (Bille, infra).

Listeria monocytogenes is a facultative intracellular pathogen, capable of growth both in the external environment and inside mammalian cells. It is responsible for opportunistic infections in both humans and animals. The first cases of human listeriosis were reported in the 1930s and outbreaks have been traced to the consumption of contaminated food, most notably dairy and poultry products (Goebel et al., 1991, Infection 19:5195-5197). Individuals at risk are the newborn, the elderly, and the immunocompromised.

Clinical features of the diseases are meningitis and meningoencephalitis. Infection with L. monocytogenes has also been observed as septicemia (with resulting abortion) in pregnant women, and patients with malignancies and immunosuppression. Some people, usually predisposed by an underlying cardiac illness, have been treated for endocarditis resulting from listerial infection.

Although L. monocytogenes is considered an uncommon adult pathogen, it is the third most common cause of bacterial meningitis in neonares (McKay & Lu, 1991, Infection & Immun. 59:4286-4290). Highest mortality and neurological sequelae among survivors is seen when the central nervous system is involved. However, underlying conditions which cause lower cell-mediated immunity, such as transplants, malignancy and AIDS, can result in increased mortality, up to 60%.

There has been a gradual increase in the incidence of human Listeriosis since the 1960s. Presumably, this is related to the increased numbers of individuals with malignancies undergoing radiation and chemotherapy, which allows for their prolonged survival but with immunosuppression as their consequence. Similarly, increases in renal transplantations has exposed increasing numbers of patients to possible infectious complications. Finally, with the rapid spread of AIDS and its suppression of immune function, it can be expected that the occurrence of human listeriosis may increase substantially in the future years.

The epithelial cells of the gastrointestinal tract may be the primary site of entry of L. monocytogenes. It was discovered in the 1960s that this bacterium can invade, survive and replicate within phagocytic cells, such as macrophages and monocytes (Michel & Cossart, 1992, J. Bacteriol. 174:7098-7103 incorporated herein by reference). Nonprofessional phagocytes, which are unable to take up extracelluarly growing bacteria, are also susceptible to invasion by this intracellular organism (Bubert et al., 1992, J. Bacteriol. 174:8166-8171 incorporated herein by reference). Apparently, L. monocytogenes is able to induce its own phagocytosis in these host cells. Specific virulence factors are required for this invasion and intracellular growth.

A major extracellular protein P60, named for its relative molecular weight of 60,000 daltons, is produced by all virulent L. monocytogenes strains. Protein P60 is derived from the Protein P60 Precursor also known as the invasion-associated protein (iap) as described by Koehler, et al., 1990, Infect. Immun. 58:1943-1950 incorporated herein by reference. Moreover, the precursor protein is 484 amino acids in length and the sequence is known.(SEQ ID NO: 26)

Spontaneously occurring mutants of L. monocytogenes that show a decreased level of the protein P60, known as R mutants, are a virulent and unable to invade nonprofessional phagocytes. R mutants are still phagocytized by macrophages with the same efficiency as wild-type bacteria and are able to replicate in these cells. Addition of partially purified P60 protein from wild-type L. monocytogenes restores the invasiveness of these R mutants into nonprofessional phagocytic cells. This finding has led to the conclusion that P60 is involved in the mechanism of uptake of L. monocytogenes by nonprofessional phagocytic cells.

The P60 protein of L. monocytogenes,(SEQ ID NO: 26)is 484 amino acids long, contains a putative N-terminal, signal sequence of 27 amino acids and an extended repeat region of 19 threonine-asparagine units. The middle portion of the protein P60, consisting of about 240 amino acids, and located about 120 amino acids from both the N-and C-terminal ends, varies considerably from the deduced amino acid sequences of the related P60 proteins of L. innocua, L ivanovii, L seeligeri, L welshimeri and L grayi. From the predicted secondary structure and hydropathy studies on this protein, the hydrophillic middle portion consists of two alpha-helical regions flanking the repeat domain. Conversely, the hydrophobic N- and C- terminal ends are in predominantly B-pleated sheets. This would suggest that the middle region is exposed on the protein's surface (Kohler, infra).

The CSF findings in listeria meningitis are quite variable and often result in a negative gram stain. This means that confirmed diagnosis is dependent on culture of either blood or CSF samples, which may take up to 48 hours. Given its high mortality and morbidity, and the increasing numbers of populations at risk, it is apparent that the need exists for rapid diagnosis and for a vaccine against L. monocytogenes infections.

It is a well known feature of bacterial and viral meningitis etiological agents that they possess the ability to infect the CNS. Until recently, it was not known how these agents could pass the Blood-Brain Barrier. The mechanism by which circulating bacteria enter the CSF compartment has only recently been understood. Circulating organisms could invade the CSF compartment by translocation through or between vascular endothelial cells and underlying tissues before entering the CSF. In fact, vascular lesions are a feature of meningitis caused by such organisms as Salmonella choleraesuls and Pasteruella haeloytica. See Wildock, 1977, Vet. Pathol 14:113-120; Sullivan, "The Nervous System: Inflammation", in Jubb et al., eds., 1985, Pathology of Domestic Animals, Volume 1:278-290 all of which are incorporated herein by reference.

However, while vascular endothelial damage may be integral to the pathogenic pathway for some bacteria, it is unlikely to be the mechanism of entry for most cases of meningitis, since vascular lesions are not a prominent early feature of meningitis caused by either N. meningtidis, S. pneumoniae, E coli, S. suls, H. parasuis, H. influenzae, or S. aureus (Williams, 1990, J. Infec. Dis., 162:474-481).

It has been shown that bacteria can be carried into the CSF in association with monocytes migrating into the CSF compartment to maintain populations of resident macrophages (Cordy, 1984, Vet. Pathol 21:593-597). This method of entry for bacteria is also analogous to the mechanism employed by some viruses (HIV, Maaedi-Visna-caprine arthritis encephalitis virus) when invading the CNS. See Peluso, 1985, Virology 147:231-236; Narayan, 1985, Rev. Infec. Dis. 7:899-98; Roy, 1988, J. Leukoc. Clol. 43:91-97; Westervelt, 1991, Vaccines 91:71-76 which are all incorporated herein by reference.

It is also known art that cellular immune reactions consist of a complex series of coordinating events. In response to tissue injury, monocytes are recruited from bone marrow via the blood circulation (Robinson, 1989, PNAS 86:1850-1854 incorporated herein by reference). These activated blood monocytes then differentiate into macrophages in response to several immune mediators produced at the site of inflammation (Yoshimura, et al., 1989, FEBS Letter 244:487-493).

As macrophages normally function to protect the body from potentially toxic substances, either infectious or chemical in nature, they serve as scavengers, processing and presenting antigen to the B lymphocytes, which in turn produce antibodies. (Edington, 1993, Bio/Technology 11:676-681 incorporated herein by reference), Macrophages and also known to secrete mediators that mediate systemic host defence responses and local inflammation.

The first evidence of mediators being involved in cellular immune reactions was noted in 1970 (Ward, 1970, Cell Immunol., 1:162-174). It was reported that addition of antigen to specifically sensitized lymphocytes caused release of an "activity" which attracted macrophages (Robinson, infra). It is now well known that immune mediators possess a variety of functions for cytokines such as the interleukins and interferons.

This led the recent discovery of a family of small, secretory cytokine-like proteins called chemokines for their apparent chemotactic properties, whose complete proinflammatory functions have yet to be elucidated. However, the size and amino acid sequence of many of these chemokines is known as illustrated in Michiel, 1993, Bio/Technology 11:739, incorporated herein in its entirety by reference.

Like most secreted proteins, the chemokines are synthesized with a hydrophobic leader sequence which is cleaved to produce the mature, active chemokine. Comparison of their amino acid sequences has shown these proteins to have a highly conserved pattern of four cysteine residues in the mature peptides. Consequently, they have been classified into two groups based on their structural characteristics: the alpha chemokine group having an intervening amino acids between cys-1 and cys-2, (ie. a C-X-C motif); the beta chemokine group has no intervening amino acid, (ie. a C-C motif). (Michiel, 1993). Cys-1 crosslinks with Cys-3 and Cys-2 crosslinks with Cys-4, resulting in a similar tertiary structure for all the proteins classified into this family of chemokines.

It is further known that the chemokines appear to be functionally involved in cell chemotaxis. Their amino acids sequence diversity suggests that each chemokine has distinct cellular specificity, each having its own unique cellular targets.(Michiel, infra). This cellular specificity appears related to seven transmembrane-domain receptors in each chemokine, but the overlapping pattern of ligand binding and their regulation has yet to be determined (Rollins, et al., 1989, Molecular & Cellular Biol. 9:4687-4695 incorporated herein by reference).

Several peptides from the beta chemokine family have been found to possess the ability for "chemo-attracting" monocytes/macrophages. One such chemotactic protein was identified in 1978 in antigen-stimulated human lymphocytes. (Robinson, infra) and was named LDCF, for Lymphocyte-Derived Chemotactic Factor. This particular chemokine has since been isolated from a variety of different glioma cell lines; human peripheral blood mononuclear leukocytes, (Yoshimura, infra); resting human monocytes (Rollins, infra); human lung fibroblasts and a primary human fetal fibroblast cell line. This latter line being the only member of the Beta family of chemokines to be identified in fibroblasts.

As with all chemokines, various names have been used to identify this protein. The following terms are therefore interchangeable for those skilled in the art: GDCF-2: for Glioma-Derived Monocyte Chemotactic Factor; hJE: for human JE gene product; MCAF: for Monocyte Chemotactic Factor; and MCP: for Monocyte Chemoattractant Protein-1. As the amino acid sequences for these chemokines was found to be identical, the term MCP has been adopted for describing this particular chemokine. It is thus referred to in the art as other chemokines that share significant sequence homology with MCP-1, and have been named MCP-2and MCP-3, according to the order of their discovery.

The amino acid sequence of MCP-1 shows the mature protein to be 99 amino acids long starting at what corresponds to nucleotide 70 of the gene. The functional portion of the protein is known to be the active portion with the first 23 amino acids serving as a signal sequence. MCP-1 is a secretory N-glycosylated glycoprotein of a variety of molecular weights but predominantly occurring at 13,000; 15,000; and 15,500 Daltons with post-translational modification probably accounting for the various forms. The two former isoforms have been named alpha and beta respectively but the structural differences between the two are still unknown. Yet, it is known that their amino acids sequences are identical, apparently derived from a single gene product.

Many mitogenic and activating stimuli appear to cause secretion of MCP-1 by a wide variety of cells. These findings suggest that the cellular regulation of MCP-1 expression is complex, and involves circulating cytokine levels in addition to other factors. Viral and bacterial infections in turn, can affect these levels and are thus involved in the function of MCP-1.

The size and amino acid sequence of MCP-3 is also known as illustrated by Van Damme, et al., 1992, J. Exp. Med. 176:59-65, incorporated herein by reference. It has also been determined that MCP-3 is a chemotactic factor that can attract monocytes and that it can bind heparin.

Amino acids sequences of all proteins described in detail in the present invention are given using the following single letter code: A=ala, C=cys, D=asp, E=glu, F=phe, G=gly, H=his, I=ile, K=lys, L=leu, M=met, N=asn, P=pro, Q=gln, Rh=arg, S=ser, T=thr, V=val, W=trp, Y=tyr.

Accordingly, there remains a significant and urgent need to determine the mechanism used by meningitis etiological agents, as diverse as bacteria and viruses, to attract and infect monocytes and/or gaining access to the CNS. There also remains a significant and urgent need to develop a therapeutic capable of blocking such infection of the CNS by bacterial and viral meningitis etiologic agents utilizing such a mechanism. Specifically, there remains a need in the art for a monoclonal antibody specific for both bacterial and viral infectious agents of meningitis, where said monoclonal antibodies would recognize both bacterial and viral infectious agents of meningitis and have substantial diagnostic utility. Additionally, there is also a need for a known proteinaceous region containing the epitope(s) recognized by said monocional antibody where said epitope or peptide could be chemically synthesized, thereby avoiding the difficulties inherent in purification and administration of larger fragments of the antigenic molecules. An additional need for this said peptide is evident for use in diagnostic test kits to indicate meningitis infection as well as would also be useful in the development of a general meningitis vaccine.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 depicts the amino acid sequence of (SEQ ID NO:1) the Structural Polyprotein protein of the M22 strain of Rubella virus with sequences of interest underlined.

FIG. 2 depicts the amino acid sequence (SEQ ID NO: 8) of the Structural Polyprotein of the Therien strain of Rubella virus with sequences of interest underlined.

FIG. 3 depicts the amino acid sequence (SEQ ID NO: 11) of the Gag Polyprotein of the LV isolate of HIV-1 with sequences of interest underlined.

FIG. 4 depicts the amino acid sequence (SEQ ID NO: 14) of the Envelope Polyprotein Precursor protein of the LV-la isolate of HIV-1 with sequences of interest underlined.

FIG. 5 depicts the amino acid sequence (SEQ ID NO: 17) of the Lipoprotein E Precursor of Haemophilus influenzae with sequences of interest underlined.

FIG. 6 depicts the amino acid sequence (SEQ ID NO: 20) of the Opacity-Related Protein of Neisseria meningitidis with sequences of interest underlined.

FIG. 7 depicts the amino acid sequence (SEQ ID NO: 23) of the Pneumococcal Surface Protein A of Streptococcus pneumoniae with sequences of interest underlined.

FIG. 8 depicts the amino acid sequence (SEQ ID NO: 26) of Protein P60 Precursor of Listeria monocytogenes with sequences of interest underlined.

FIG. 9 depicts the amino acid sequence (SEQ ID NO: 35) of the chemokine hMCP-1 with sequences of interest underlined.

FIG. 10 depicts the amino acid sequence (SEQ ID NO: 38) of the chemokine HMCP-3 with sequences of interest underlined.

FIG. 11, Immunoblots of RV antigens reacted with Mab's RV1, RV2, RV3 and RV4. RV antigen: Strain MPV-77 (lot #50678, Catalogue #EL-05-04) cultured in Vero cells. Purchased from Microbix Biosystems Inc., Toronto, Ontario). All Mab used as tissue culture fluid diluted 1/500. Lane 1 - Molecular weight markers of 97, 66, 45, 31, 21, and 14kD. Lane 2/3 - RV4; lane 4/5/6 - RV3; lane 7/8 - RV2; lane 9/10 - RV1. Lanes 2-9 all illustrate two proteins, 31 kD (major) and 45 kD (minor), identified by reactions with Mab's 1-4.

FIG. 12, Immunoblots of bacterial antigens reacted with V Mab RV1. H. Influenzae b antigen from ATCC (#10211); L. monocytogenes from ATCC (#7644); S. pneumoniae from the Caribbean Regional Epidemiology Centre, CAREC, Trinidad; N. meningitidis A from ATCC (#13077).

Lane 1-Molecular weight markers of 97, 66, 45, 31, 21, and 14 kD. Lane 2-H. Influenzae b-proteins of approximate weights of 50, 45, 40, and 25 kD. Lane 3-L. monocytogenes-proteins of approximate weights of 60 kD (major) and 66 kD (minor), Lane 4/5 -S. pneumoniae-proteins of approximate weights of 60 kD and 66 kD, Lane 6/7 -N. meningitidis-a protein of approximate weights of 18 kD, identified by reaction with Mab RV1.

FIG. 13, Immunoblots of HIV1 antigens reacted with RV Mab RV1. HTLV-IIIB viral lysate, lot #54.-040, purchased from Applied Biotechnologies, Inc., Md., USA. Lane 1 - Molecular weight markers of 97, 66, 45, 31, 21, and 14 kD. Lane 2 - Control RV antigens, 31 and 45 kD, reacting with RV1 Mab. Lane 3/4 - HIV1 antigen of approximate weights of proteins at 24 kD and 61 kD, identified by reaction with Mab RV1.

BRIEF DESCRIPTION OF THE INVENTION

The present invention provides novel peptides corresponding to homologous antigenic amino acid sequences on regions of bacterial and viral agents known to cause meningitis and on chemokines known to attract monocytes, in addition to monoclonal antibodies reactive with such antigenic regions and peptides. It also provides analogues of those peptides and mixtures and combinations of those peptides and analogues. These novel materials find use in, for example, a wide variety of diagnostic and preventive methods, means and compositions with respect to the overall process of pathogenesis which uses chemokine function to promote disease including meningitis, and atherosclerosis.

DETAILED DESCRIPTION OF THE INVENTION

The present invention provides novel compositions and methods for detecting, preventing and therapeutically treating disease wherein the pathogen or pathogenic mechanism includes a monoclonal antibody defined antigenic sequence. More specifically, using a monoclonal antibody defined by two rubella virus antigenic sites, a family of homologous cross-reacting antigenic sequences were identified in proteins associated with meningitis etiologic agents. These cross reacting antigenic sequences were in turn found to be significantly homologous to the C-terminal sequence of the monocyte attracting chemokines hMCP-1 and hMCP-3. Hence, this invention involves the use of peptides that mimick these homologous cross-reacting antigenic sequences and monoclonal antibodies reactive with such amino acid sequences to diagnose, treat and vaccinate against diseases wherein the pathogenic mechanism involves one or more members of these homologous cross-reacting sequences. An example of such a disease is meningitis.

A monoclonal antibody was used to identify two cross-reacting septapeptide antigens (QPQPPRM and PPQPPRA SEQ ID NO: 3 and 7 Respective) contained in the Structural Polyprotein (Core and E2 outer membrane proteins portion described in greater detail below) of Rubella virus. The monoclonal antibody, RV1-Mab, was also found to cross-react with the p24 core protein and the p61 outer membrane protein of Human Immunodeficiency Virus-1 (HIV-1), known to cause meningitis during the initial stages of infection. Furthermore, the RV1-Mab was also found to cross react with proteins found in Hemophilus influenzae, Neisseria meningitidis, Streptococcus pneumoniae and Listeria monocytogenes, which together account for more than 85% of all bacterial meningitis in the United States. In this way, a family of homologous cross-reacting septapeptide antigens were discovered in viruses and bacteria known to cause meningitis. Because the RV1-Mab binds to amino acid sequences in diverse bacteria and viruses that are related only in the fact that they cause meningitis, these closely related homologous sequences have been designated Meningitis Related Homologous Antigenic Sequence (MRHAS).

A member of the MRHAS family can be defined as an amino acid sequence that is homologous to antigenic sites on the Structural Polypeptide (within the core and E2 membrane protein portion) of Rubella virus that are recognized by a Mab from the hybridoma RV-1. More specifically, any amino acid sequence, that is homologous to the regions extending from approximately amino acid residue 102 to 108 of the Structural Polyprotein (core protein region) and from about 313 to 319 of the Structural Polyprotein (E2 membrane protein) of the M33 strain of Rubella virus is by definition a member of the MRHAS family of sequences. The complete sequence of this Structural Polyprotein is found in FIG. 1 (SEQ ID NO: 1)Representative members that are cross-reactive with the RV1-Mab and appear in bacteria and viruses known to cause meningitis are presented in Table 1. The sequences of the proteins listed in Table 1 are found in FIGS. 1-8 and in SEQ ID Nos. 3, 5, 7, 10, 13, 16, 19, 22, 25, 28, 30, 32, and 34.

                  TABLE 1     ______________________________________               VIRUS/     NAME      BACTERIUM   PROTEIN     SEQUENCE     ______________________________________     MRHASRV-1 Rubella     Structural  QPQPPRM               virus       Polyprotein                           (Core)     MRHASRV-2 Rubella     Structural  QTPAPKP               virus       Polyprotein                           (Core)     MRHASRV-3 Rubella     Structural  PPQPPRA               virus       Polyprotein                           (E2)     MRHASRV-4 Rubella     Structural  LPQPPCA               virus       Polyprotein                           (E2)     MRHASHIV-1               HIV1        Gag         QAISPRT                           Polyprotein     MRHASHIV-2               HIV1        Envelope    QNQQEKN                           Polyprotein                           Precursor     MRHASHI-1 Hemophilus  Lipoprotein QVQNNKP               influenzas  E Precursor     MRHASNM-L Nisseria    Opacity-    IQPPKN               meningitidis                           Related                           Protein POPM3     MRHASSP-1 Streptococcus                           Pneumococcal                                       QQQPPKA               pneumonias  Surface                           Protein A     MRHASLM-1 Listeria    Protein     PTQEVKK               monacytogenes                           P60                           Precursor     MRHASLM-2 Listeria    Protein     TTPAPKV               monocytogenes                           P60                           Precursor     MRHASLM-3 Listeria    Protein     NTATPKA               monocytogenes                           P60                           Precursor     MRHASLM-4 Listeria    Protein     QQTAPKA               monocytogenes                           P60                           Precursor     ______________________________________

It is noted that within the Structural Polyprotein of Rubella virus, there are three proteins that can be ultimately derived. Therefore, when a reference is made to either the Core protein portion or the E2 membrane-associated protein portion (from either the M33 or Therien strains), this reference denotes the portion of the Structural Polyprotein from which the final mature protein will be derived. A similar nomenclature with respect to precursor versus mature protein was also used in connection with the Gag Polyprotein of HIV-1, the Envelope Polyprotein Precursor of HIV-1, the Lipoprotein E Precursor, and the Protein P60 Precursor. For example the Protein P60 Precursor has, at a minimum, a 27 amino acid leader sequence that is removed during processing to mature protein.

Members of the MRHAS family were also found to appear in two variants of the chemokine, human Monocyte Chemoattractant Factor (hMCF). These two are hMCP-1 and hMCP-3, as indicated in Table 2. The sequences of the factors listed in Table 2 are found in FIGS. 9 and 10 SEQ ID NOS 35 and 38, respectively) and in SEQ ID NOS 37 and 40.

                  TABLE 2     ______________________________________     NAME       FACTOR     POSITION    SEQUENCE     ______________________________________     MRHASMCP-l hMCP-l     70-76       OTOTPKT     MRHASMCP-3 hMCP-3     61-67       KTQTPKL     ______________________________________

It is surprising that bacteria and viruses as diverse as Hemophilus influenzae, Neisseria meningitidis, Streptococcus pneumonias, Listeria monocytogenes, RV, and HIV-1 share a common feature, namely the placement of MRHAS, a highly conserved sequence, on the outer membrane. However, some of these etiological agents of meningitis do share specific features. For example, Williams and Blakemore have shown that bacteria can be carried into the CNS in association with monocytes migrating into the CSF compartment to maintain populations of resident macrophages (Cordy, 1984, vet. Pathol. 21:593-597). This method of entry for bacteria would be analogous to that by which some viruses (HIV, Maaedi-Visna-caprine arthritis encephalitis virus) invade the CNS (Peluso, et al., 1985, Virology 147:231-236; Narayan and Cork, 1985, Rev. Infec. Dis. 7:899; Roy and Wainberg, 1988, J. Leukoc. Clol. 43:91-97; Westervelt et al., 1991, Vaccines 91:71-76). Moreover, available information for HIV-1 indicates that significant alterations in proteins carrying the MRHAS alters virulence, or invasiveness of the organisms.

Since the MRHAS that appear on bacterial and viral organisms are significantly homologous to sequences found in monocyte attracting chemokines, it is apparent that these agents have incorporated these sequences into their proteins to attract monocytes to aid in infection.

The unexpected discovery of monoclonal antibody cross-reactivity over various viral and bacterial species known to cause meningitis provides novel means for therapeutic and prophylactic treatments of meningitis. Moreover, the utility of this invention is extended by the significant homology of these antigenic sites with amino acid sequences in monocyte attracting chemokines. These novel means may be applied to dieases as diverse as meningitis and atherosclerosis, wherein the pathogen or pathogenic mechanism includes one or more of these MRHAS.

More specifically, a hybridoma is used to produce cross-reacting monoclonal antibodies that bind MRHAS in vivo and in vitro. These antibodies are useful as a diagnostic tool to detect the presence of MRHAS. One such diagnostic use is to detect the presence of bacterial and viral agents of meningitis in biological samples. Such Mabs are also useful for treating a patient to prevent and/or treat infection due to a meningitis etiologic virus and/or bacteria. A bacterial and/or viral meningitis infection can also be detected using peptides mimicking MRHAS in a diagnostic test. In vivo, peptides mimicking MRHAS can also be used as a novel vaccine for meningitis, in addition to use as blocking agents (therapeutics) to prevent the accumulation of monocytes involved in CNS infection and diseases such as atherosclerosis.

In one aspect, the novel peptides, typically less than about 30 amino acids, contain seven or more contiguous amino acids forming epitopes substantially similar to epitopes located on viruses and/or bacteria known to cause meningitis and/or on chemokines known to attract monocytes. Of particular interest are the regions extending from about amino acid residue: 102 to 108 (SEQ ID NO: 3) (core protein portion), 89 to 95 (SEQ ID NO: 5)(core protein portion), and 313 to 319 (SEQ ID NO: 7)(52 membrane portion) of the Structural Polyprotein of the M33 strain of Rubella virus; from about 314 to 320 (SEQ ID NO: 13)(E2 membrane portion) of the Structural Polyprotein of the Therien strain of Rubella virus; from about 145 to 151 of the Gag Polyprotein of the LV isolate of HIV-1; from about 655 to 661 (SEQ ID NO: 16) of the Envelope Polyprotein Precursor of the LAV-la isolate of HIV-1; from about 99 to 105 )SEQ ID NO: 22) of the Lipoprotein E Precursor of Haemophilus influenzae; from about 1 to 5 of the Opacity-Related Protein POPM3 of Neisseria meningitidis; from about 423 to 429 (SEQ ID NO: 25) of the Pneumococcal Surface Protein A of Streptococcus pneumoniae; from about 151 to 157, (SEQ ID NO: 2) 181 to 187, (SEQ ID NO: 30) 249 to 255,(SEQ ID NO: 32) and 292 to 298 (SEQ ID NO: 34) of the Protein P60 Precursor of Listeria monocytogenes; from about 93 to 99 (SEQ ID NO: 37) of the chemokine hMCP-1; and from about 61 to 67 of the chemokine hMCP-3.

Those skilled in the art will appreciate that additional analogous regions ("homologs") from other infectious agents (viruses, bacteria, etc.) or chemokines may be identified based upon their sequence homology with members of the MRHAS family. In practice, such homologs may be identified by reference to the MRHAS ocurring in hMCP-1, QTQTPKT (SEQ ID NO: 37).

This method can be applied to other infectious agents (viruses, bacteria, etc.) or chemokines that are yet to be discovered. For example, as new viruses or bacteria are identified that use monocytes to infect various regions of the body such as the CNS, their protein amino acid sequences may be aligned with that of the MRHAS in hMCP-1 to obtain maximum homology. The methods by which the sequences are aligned are known to those skilled in the art. The amino acid sequence of an infectious agent not listed herein, which corresponds to members of the MRHAS family specifically disclosed herein can be synthesized and used in accordance with the invention.

It is not necessary to the present invention that the epitopes contained within such sequences be cross-reactive with antibodies to all infectious agents of meningitis, or all chemokines that attract monocytes. Peptides encompassing immunological eiptopes which distinguish between types of monocytes or between efficacity for a particular type of monocyte will find utility in identifying different pathogenic mechanisms of infection and disease. For example, such utility will include infectious agents that use different modes of infectivity to enter the CNS. These peptides may also be useful in combination with other peptides representing other members of the MRHAS family in therapeutic composition.

In accordance with another aspect of the present invention, a novel cell line capable of producing monoclonal antibodies and compositions comprising such antibodies is provided, which antibodies are capable of selectively recognizing members of the MRHAS family. These monoclonal antibodies may be used in a wide variety of ways including diagnosis and therapy, as well as to identify other cross-reactive antibodies. Peptides or polypeptides containing the epitope(s) with which they react may find separate uses as immunogens for vaccines, or as therapeutic agents.

Generation of Monoclonal Antibodies

Monoclonal antibodies were prepared by immortalizing the expression of nucleic acid sequences that encode for antibodies or binding fragments thereof specific for members of the MRHAS family. See Godding, 1980, "Antibody Production by Hybridomas", J. Immunol. Meth., 39:285-308 which is incorporated herein by reference. In brief, spleen cells from an immunized vertebrate that illustrate the desired antibody response are immortalized. Immunization protocols are well established and though such protocols can be varied considerably, they still remain effective. Also see, Goding, 1986, Monoclonal Antibodies: Principles and Practice, Academic Press, 2nd edition, which is herein incorporated by reference. Cell lines that produce the antibodies are most commonly made by cell fusion between suitably drug-marked human or mouse myeloma or human lymphoblastoid cells with human B-lymphocytes to yield the hybrid cell lines. Other methods include Ebstein-Barr Virus transformation of lymphocytes, transformation with bare DNA (such as oncogenes or retroviruses), or any other method which provides for stable maintenance of the cell line and the production of monoclonal antibodies. The general methodology followed for obtaining monoclonal antibodies is described in Kohler & Milstein, 1975, Nature. 256:495-496, which is incorporated herein by reference. The transformation or fusion can be carried out in conventional ways, the fusion technique being described in a number of patents: U.S. Pat. Nos. 4,172,124; 4,350,683: 4,363,799; 4,381,292; and 4,423,147, whose techniques and technologies are herein incorporated by reference. The procedure is also described by Kennett et al., Monoclonal Antibodies (1980) and references therein, as well as Goding, infra, all of which are incorporated herein by reference. Human monoclonal antibodies are acquired by fusion of the spleen cells with the appropriate human fusion partner, such as WI-L2 and as described in European Application No. 82,301103.6, the relevant portions of such a procedure incorporated herein by reference. A detailed technique for producing mouse X mouse monoclonal antibodies is taught by Oi & Herzenberg, in Mishell & Shiigi, 1980, Selected Methods in Cellular Immunology:351-372, which also is incorporated herein by reference. The resulting hybridomas are screened to isolate individual clones, where each clone secretes a single monoclonal antibody to a given MRHAS.

The antibodies generated herein can be used without modification or may be modified in a number of ways. For example, such modification can be by way of labeling (meaning joining), either covalently or non-covalently, a moiety which directly or indirectly provides for some means of detection. A variety of such labels are known and include: substrates, enzymes, co-factors, inhibitors, chemiluminescers, fluorescers, radionuclides, magnetic particles, and the like.

Moreover, fragments of such monoclonal antibodies can exist that continue to possess noteable specificity for a given MRHAS. As such, all antibody binding fragments or reference to such `fragaent(s) thereof` refers to a lesser portion of a complete antibody that retains some, if not all, of its binding specificity and capacity for a given MRHAS.

Therefore, one preferred embodiment of this invention involves a composition comprising a monoclonal antibody or binding fragment thereof which binds to one or more members of a group of homologous antigenic amino acid sequences comprising MRHAS.

A further embodiment of this invention involves a cell line that produces a monoclonal antibody or binding fragment thereof which binds to members of a family comprising MRHAS. As yet another embodiment of this invention involves a cell line that produces a monoclonal antibody or binding fragment thereof which binds to an epitope shared by bacterial and viral meningitis etiologic agents, wherein said cell line is RV-1 which was deposited at the American Type Tissue Collection (ATCC) Rockville, Md, on May 26, 1993 and accorded accession number HB 11362.

Another embodiment of this invention is a monoclonal antibody produced by the cell line RV-1 (ATCC HB 11362).

It is also a preferred embodiment of this invention that there be a monoclonal antibody capable of reacting with a MRHAS, wherein the monoclonal antibody specifically blocks the binding of an antibody produced by a cell line that produces a monoclonal antibody or binding fragment thereof which binds to members of a family comprising MRHAS, and where such cell line can be RV-1 (ATCC HB 11362).

Another embodiment involves a monoclonal antibody capable of reacting with an antigenic determinant, or homologs thereof, wherein the monoclonal antibody specifically blocks the binding of an antibody produced by a cell line that produces a monoclonal antibody or binding fragment thereof which binds to members of a family comprising MRHAS, and where said cell line can be RV-1 (ATCC HB 11362) and wherein said antigenic determinant is selected from the amino acid sequences presented in Table 3 and in SEQ ID NOS 2, 4, 6, 9, 12, 15, 18, 21, 24, 27, 29, 31, 33, 36 and 39.

                                      TABLE 3     __________________________________________________________________________     VIRUS/     BACTERIUM/      AMINO ACID                             AMINO ACID     CREMORINE             PROTEIN REGION  SEQUENCE     __________________________________________________________________________     Rubella Structural                     95-115  PSRAPPQQPQPPRMQTGRGGS     virus   Polyprotein     Rubella Structural                     82-102  ERQESRSQTPAPKPSRAPPQQ     virus   Polyprotein     Rubella Structural                     306-326 DMAAPPMPPQPPRAHGQHYGH     virus   Polyprotein     Rubella Structural                     306-326 DMAAPPTLPQPPCAHGQHYGH     virus   Polyprotein     HIV-1   Gag     138-158 IQGQMVHQAISPRTLNAWVKV             Polyprotein     HIV-1   Envelope                     648-668 HSLIEESQNQQEKNEQELLEL             Polyprotein             Precursor     Haemophilus             Lipoprotein                     92-111  NSPYAGWQVQNNKPFDGKDWT     influenzae             E Precursor     Neisseria             Opacity-                     1-13    IQPPKNLLFSSLL     meningitidis             Related             Protein POPM3     Streptococcus             Pneumococcal                     416-436 EEYNRLTQQQPPKAEKPAPAP     pneumoniae             Surface             Protein A     Listeria             Protein 144-164 AVSTPVAPTQEVKKETTTQQA     monocytogenes             P60             Precursor     Listeria             Protein 174-194 VKQTTQATTPAPKVAETKETP     monocytogenes             P60             Precursor     Listeria             Protein 242-262 LAIKQTANTATPKAEVKTEAP     monocytogenes             P60             Precursor     Listeria             Protein 285-305 KKETATQQQTAPKAPTEAAKP     monocytogenes             P60             Precursor     Chemokine       86-99   SMDHLDKQTQTPKT     hMCP-l     Chemokine       54-67   FMKHLDKKTQTPKL     hMCP-3     __________________________________________________________________________

Yet another embodiment of this invention is a monoclonal antibody capable of reacting with an antigenic determinant of the proteins presented in Table 4, wherein the antigenic determinant is selected from the amino acid sequences presented in Table 1 and in SEQ ID NOS 3, 5, 7, 10, 13, 16, 19, 22, 25, 28, 30, 32, 34, 37 and 40.

                  TABLE 4     ______________________________________     VIRUS/                            AMINO     BACTERIUM/            AMINO ACID  ACID     CREMOKINE PROTEIN     REGION      SEQUENCE     ______________________________________     Rubella   Structural  102-108     QPQPPRM     virus     Polyprotein     Rubella   Structural  89-95       QTPAPKP     virus     Polyprotein     Rubella   Structural  313-319     PPQPPRA     virus     Polyprotein     Rubella   Structural  313-319     LPQPPCA     virus     Polyprotein     HIV-1     Gag         145-151     QAISPRT               Polyprotein     HIV-1     Envelope    655-661     QNQQEKN               Polyprotein               Precursor     Haemophilus               Lipoprotein  99-105     QVQNNKP     influenzae               E Precursor     Neisseria Opacity-    1-5         IQPPKN     meningitidis               Related               Protein POPM3     Streptococcus               Pneumococcal                           423-429     QQQPPKA     pneumoniae               Surface               Protein A     Listeria  Protein     151-157     PTQEVKK     monocytogenes               P60               Precursor     Listeria  Protein     181-187     TTPAPKV     monocytogenes               P60               Precursor     Listeria  Protein     249-255     NTATPKA     monocytogenes               P60               Precursor     Listeria  Protein     292-298     QQTAPKA     monocytogenes               P60               Precursor     Chemokine             93-99       QTQTPKT     hMCP-l     Chemokine             61-67       KTQTPKL     hMCP-3     ______________________________________

Pharmaceutical Formulations and Use

The monoclonal antibodies of this invention that bind MRHAS can also be incorporated as components of pharmaceutical compositions. The composition should contain a therapeutic or prophylactic amount of at least one of the monoclonal antibodies of the present invention with a carrier that is pharmaceutically effective. Such a pharmaceutical carrier should be any compatible, non-toxic substance that is suitable to deliver the monoclonal antibodies to the patient. Such carriers can be sterile water, alcohols, fats, waxes, and inert solids. The pharmaceutical composition may also be incorporate pharmaceutically acceptable adjuvants (eg. buffering agents or dispersing agents). Hence, the monoclonal antibodies of the present invention can be employed as separately administered compositions given in conjunction with other anti-bacterial or anti-viral agents.

The monoclonal antibodies, peptides, and pharmaceutical compositions thereof, of the present invention are particularly useful for oral or parenteral administration. It is preferred that the pharmaceutical compositions be administered parenterally: i.e., subcutaneously, intramuscularly, or intravenously. Therefore, this invention is providing compositions for parenteral administration that comprises a solution of the monoclonal antibody, peptide, or a cocktail thereof dissolved in an suitable carrier (which is preferably an aqueous carrier). Examples of the aqueous carriers that can be used are water, buffered water, 0.4% saline, 0.3% glycine, or the like. These solutions are to be sterile and generally free of particulate matter: Moreover, these compositions may be sterilized by conventional and well known sterilization techniques. The compositions may also contain pharmaceutically acceptable auxiliary substances. These substances are required to approximate physiological conditions such as pH adjusting and buffering agents, toxicity adjusting agents, and the like. Examples of these auxiliary substances are sodium acetate, sodium chloride, potassium chloride, calcium chloride, sodium lactate, etc. The concentration of antibody and/or peptide in these formulations can widely vary depending on its ultimate use, activity, and mode of administration of the composition. The concentration of antibody and/or peptide in these formulations will be selected primarily based on such factors as fluid volumes, viscosities, etc. It is preferable that such factors be chosen for the partiuclar mode of administration selected. The actual methods used for preparing parenterally administrable compositions will be known or is apparent to those skilled in the art and are described in Remington's Pharmaceutical Science, 15th Ed. (Easton: Mack Publishing Company, 1980), which is herein incorporated by reference.

The monoclonal antibodies and peptides of this invention can be lyophilized for storage and can be reconstituted in a suitable carrier prior to their use. Such techniques have been shown to be effective with conventional immunoglobulins and lyophilization and reconstitution techniques that are known in the art can be applied. It will also be appreciated by those skilled in the art however, that lyophilization and reconstitution can lead to varying degrees of antibody activity loss (e.g., with conventional immunoglobulins, IgM antibodies tend to have greater activity loss than IgG antibodies). As such, the use levels may have to be adjusted to compensate for any possible loss of activity.

The compositions containing the present monoclonal antibodies, or cocktails thereof can be dispensed for the prophylactic and/or therapeutic treatment of such diseases as meningitis or other maladies that may involve monocytes, monocyte-attracting chemokines or MRHAS (such as arteriosclserosis). In such therapeutic application, compositions are administered to patients who have contracted or begun to develop a disease involving MRHAS, chemokines, or chemokine recognizing monocytes in the pathogenic mechanism. The administration of such composition is in an amount sufficient to bind the chemical signal, i.e. to the MRHAS or chemokine. For example, a composition comprising the present monoclonal antibody is administered in a therapeutic application to a patient--already infected with a meningitis etiologic agent(s)--in an amount sufficient to cure, arrest, or at least partially arrest the infection and its complications.

In prophylactic applications, compositions containing the present antibodies or a cocktail thereof are administered to a patient not already infected by a disease-causing agent bearing an antigen that contains a MRHAS (ie. a meningitis-causing agent), but perhaps such patient has recently been exposed to or thought to have been exposed to, or was at risk of being exposed to such agent, to enhance the patient's resistance to such potential infection or to vaccinate against such agent.

The compositions containing the present peptides or cocktails thereof can be administered not only for the prophylactic and/or therapeutic treatment of meningitis, but also possibly for arteriosclserosis, or such related disease involving monocytes, monocyte-attracting chemokines or MRHAS. In therapeutic application, compositions are administered to a patient who has contracted or begun to develop a disease involving MRHAS, or homologs thereof, or chemokine recognizing monocytes in the pathogenic mechanism, in an amount sufficient to block the MRHAS signal recognition by monocytes. For example, a composition containing such a peptide may be administered in a therapeutic application to a patient already infected with a meningitis etiologic agent(s), in an amount sufficient to block MRHAS recognition sites on monocytes by interfering with the ability of said agents to attract and infect monocytes (and thus interfere with the infectivity of the CNS by said agent(s).

In prophylactic applications, compositions containing one or more peptides mimicking members of the MRHAS family or a cocktail thereof are also useful as the active component of vaccines capable of inducing protective immunity against both bacterial and vital meningitis causing agents. The possible routes of administration, the antigen doses, and the number and frequency of injections will vary from individual to individual and may parallel those currently being used in providing immunity to other viral infections. For example, the vaccines of the present invention are pharmaceutically acceptable compositions that contain at least one peptide of this invention, its analogues or mixtures or combinations thereof, in an amount that is effective in a mammal (including humans) treated with that composition to raise antibodies sufficient to protect such mammal from viral or bacterial meningitis for a period of time.

The vaccines of the present invention are prepared in accordance with known methods and are conveniently and conventionally combined with physiologically acceptable carrier materials, such as pharmaceutical grade saline, tetanus toxoid, and keyhole limpet hemocyanin. The vaccine compositions of the present invention may also include adjuvants or other enhancers of immune response, such as liposomes, alum preparations, or immunomodulators. Furthermore, these vaccine compositions may comprise other antigens to provide immunity against other viruses and bacteria. The amount of these other antigens is again dependent on the mammal to be treated, the type of disease, and the actual course of the disease. A single or multiple administration of the compositions can be done with dose levels and pattern being selected by the administering physician. However, the antigen should be present in an amount effective to raise antibodies sufficient to protect the treated mammal from that pathogen or virus for a period of time.

Furthermore, the monoclonal antibodies of the present invention may find use as a target-specific carrier molecule. Such use would involve binding an antibody to either a toxin to form an immunotoxin, or radioactive material or drug to form a radiopharmaceutical or pharmaceutical. Methods for producing immunotoxins, radiopharmaceuticals, or such pharmaceuticals are well known as set out in 1984, Cancer Treatment Reports 68:317 which is incorporated herein by reference.

It is also possible that heteroaggregates of the monoclonal antibodies from the present invention and human T-cell activators (such as monoclonal antibodies to the CD3 antigen or to the Fc gamma receptor on T-cells) may enable human T-cells or Fc-gamma bearing cells (such as K cells or neutrophils) to kill meningitis-etiologic agent infected cells via antibody dependent cell-mediated cytolysis. By way of example, such heteroaggregates may be assembled by covalently cross-linking the anti-MRHAS antibodies to the anti-CD3 antibodies using the heterobifunctional reagent N-succinimidyl-3-(2-pyridyldithiol)-propionate, as described by Karpowsky et al., 1984, J. Exp. Med. 160:168, which is herein incorporated by reference.

It is therefore, a preferred embodiment of this invention that there be a monoclonal antibody composition specifically reactive listed in Table 3 (SEQ ID NOS 2, 4, 6, 9, 12, 15, 18, 21, 24, 27, 29, 31, 33, 36 and 39), wherein the sequence or homolog of said sequence is within the region listed in Table 3, and wherein said monoclonal antibody is capable of blocking the infectivity of the virus or bacteria.

A further embodiment of this invention involves a monoclonal antibody composition specifically reactive with an epitope of a chemokine selected from one of the chemokine sequences listed in Table 4 (SEQ ID NOS 3, 5, 7, 10, 13, 16, 19, 22, 25, 28, 30, 32, 34, 37 and 40) wherein the sequence or homolog of said sequence is within the region listed in Table 3 (SEQ ID NOS 4, 6, 9, 12, 15, 18, 21, 24, 27, 29, 31, 33, 36 and 39) and wherein said monoclonal antibody is capable of binding said chemokine in vivo to significantly reduce CNS infectivity of meningitis etiologic agents.

Yet another embodiment of this invention is a vaccine formulation comprising an immunogenic peptide comprising one or more members of the MRHAS family or an immunogenic portion thereof.

Another embodiment of this invention is a method for protecting against CNS infection of bacterial and/or viral meningitis etiologic agents by blocking a recognition site on monocytes that recognizes MRHASs.

A further embodiment of this invention is a method of treating a patient to prevent an infection due to a meningitis etiologic virus and/or bacteria, said method comprising administering a prophylactically effective amount of a composition useful in the prophylactic or therapeutic treatment of viral and/or bacterial meningitis, said composition comprising a monoclonal antibody or binding fragment thereof which binds to MRHAS shared by viral and/or bacterial meningitis etiologic agents.

Yet another embodiment of this invention is a method of treating a patient infected with a meningitis etiologic virus and/or bacteria, said method comprising administering a therapeutically effective amount of a composition useful in the prophylactic or therapeutic treatment of viral and/or bacterial meningitis, said composition comprising a monoclonal antibody or binding fragment thereof which binds to MRHAS shared by viral and/or bacterial meningitis etiologic agents.

Another embodiment of this invention entails an article of manufacture adapted for use in an immunoassay for antibodies to bacterial and/or viral meningitis etiologic agents comprising a solid support having bound thereto a peptide comprising one or more members of a group of peptides based on MRHASs, wherein said peptide having the formula a---X---b, wherein X is a sequence of at least 7 amino acids taken as a block selected from the group comprised in Table 5 below, with said block maintaining the sequence in the N terminus to C terminus direction of the native amino acid sequence and analogue thereof, said analogues resulting from conservative substitutions in or modifications to the native amino acid sequence block;

a is selected from the group consisting of:

(i) an amino terminus;

(ii) one to eight amino acids taken as a block from and maintaining the sequence and N terminus to C terminus direction of that portion of the native amino acid sequence of the protein immediately N-terminal to said X or conservative substitutions in or modifications thereto; and

(iii) a substituent effective to facilitate coupling of the peptide to another moiety; and

b is selected from the group consisting of:

(i) a carboxy terminus;

(ii) one to eight amino acids taken as a block from and maintaining the sequence and N terminus to C terminus direction of that portion of the native amino acid sequence of the protein immediately C-terminal to said X or conservative substitutions in or modifications thereto; and

(iii) a substituent effective to facilitate coupling of the peptide to another moiety.

A further embodiment of the present invention is a composition useful in the prophylactic or therapeutic treatment of viral and/or bacterial meningitis, said composition comprising peptides selected from the MRHAS family and/or the peptides described in the preceeding paragraph.

Diagnostic Uses of Monoclonal Antibodies

The monoclonal antibodies and peptides of the present invention are also useful for diagnostic purposes and can be either labelled or unlabelled. Diagnostic assays typically entail the detection of a complex formation through the binding of the monoclonal antibody to a MRHAS. When unlabelled, the antibodies can find use, for example, in agglutination assays. Moreover, unlabelled antibodies can be used in combination with other labelled antibodies (second antibodies) that are reactive with the monoclonal antibody of the present invention. An example of this is antibodies specific for immunoblobulin. Alternatively, the monoclonal antibodies can be directly labelled. A wide variety of labels may be employed, such as enzymes, enzyme substrates, enzyme cofactors, enzyme inhibitors, radionuclides, fluorescers, ligands (particularly haptens), etc. In addition, numerous types of immunoassays are available and, by way of example, some assays include those described in U.S. Pat. Nos. 3,817,827; 3,850,752; 3,901,654; 3,935,074; 3,984,533; 3,996,345; 4,034,074; and 4,098,876, all of which (with references) are incorporated herein by reference.

It is common for the monoclonal antibodies and peptides of the present invention to be employed in enzyme immunoassays, where for example, the subject antibodies (or second antibodies from a different species) are conjugated to an enzyme. When a biological sample containing MRHAS antigens, such as human blood serum, saliva, cerebrospinal fluid or bacterial and/or viral infected cell culture suspension, is combined with the subject antibodies, binding occurs between the antibodies and those molecules exhibiting the desired epitope. It should be noted that the biological sample may require concentration in order to detect organisms of low titer. Such proteins, bacterial or viral particles may then be separated from any unbound reagents and a second antibody (labeled with an enzyme) added. The presence of the antibody-enzyme conjugate specifically bound to the antigen can then be determined. Other conventional techniques well known to those skilled in the art may also be used.

Kits can also be equipped with the subject monoclonal antibodies of the present invention, for detection of meningitis etiologic agents or for the presence of MRHASs. Hence, the subject monoclonal antibody compositions of the present invention may be provided, usually in a lyophilized form, either alone or in conjunction with additional antibodies specific for other epitopes of meningitis etiologic agents. The antibodies, which may be conjugated to a label, or unconjugated, are included in such kits along with buffers such as Tris, phosphate, carbonate, and the like, along with the requesite stabilizers, biocides, inert proteins (eg. bovine serum albumin) that are standard to those skilled in the art.

It is therefore, a preferred embodiment of this invention that there be a monoclonal antibody composition specifically reactive with an epitope selected from one the bacterial or viral sequences listed in Table 3 (SEQ ID NOS 2, 4, 6, 9, 12, 15, 18, 21, 24, 27, 29, 31, 33, 36 and 39) wherein the sequence or homolog of said sequence is within the region listed in Table 3, and wherein said monoclonal antibody is capable of detecting the infectivity of the virus or bacteria. As a note, that use of the said antibodies with biological samples containing low titer meningitis etiologic agents may require concentrating said samples before the diagnostic procedure is performed.

A further embodiment involves a monoclonal antibody composition specifically reactive with an epitope selected from one of the chemokine sequences listed in Table 3 (SEQ ID NOS 2, 4, 6, 9, 12, 15, 18, 21, 24, 27, 29, 31, 33, 36 and 39) wherein the sequence or homolog of said sequence is within the region listed in Table 3, and wherein said monoclonal antibody is capable of detecting said chemokine in vivo to indicate CNS infectivity of meningitis causing agents.

Yet another embodiment of this invention entails a method of diagnosing the presence of bacterial and/or viral meningitis etiologic agents in a biological sample, said method comprising the steps of forming an antibody/antigen complex wherein the antibody portion of said complex comprises a monoclonal antibody capable of binding to both bacterial and vital meningitis etiologic agents, and detecting the presence of the antibody/antigen complex formed.

A further embodiment of this invention involves an immunoassay to detect the presence of antibodies to bacterial and/or viral meningitis etiologic agents in a biological sample comprising contacting said sample with one or more immunogenic peptide(s), where said peptide is selected from one or more members of the MRHAS family, the improvement comprising the method of screening for bacterial and/or viral meningitis etiologic agents in one test.

A further embodiment of this invention involves an immunoassay to detect the presence of antibodies to bacterial and/or viral meningitis etiologic agents in a biological sample comprising contacting said sample with one or more immunogenic peptide(s), where said peptide is selected from one or more members of the MRHAS family comprising a peptide having the formula

    a---X---b

wherein:

X is a sequence of at least 7 amino acids taken as a block selected from the group comprised in Table 5:

TABLE 5

(i) the amino acid sequence of the Structural Polyprotein of a strain of Rubella virus that corresponds to AA₁₀₂ -AA₁₀₈ (SEQ ID NO: 3) of said protein of the M₃₃ strain of Rubella virus as set forth in FIG. 1 (SEQ ID NO: 1):

(ii) the amino acid sequence of the Structural Polyprotein of a strain of Rubella virus that corresponds to AA₈₉ -AA₉₇ (SEQ ID NO: 5) of said protein of the M33 strain of Rubella virus as set forth in FIG. 1 (SEQ ID NO: 1)

(iii) the amino acid sequence of the Structural Polyprotein of a strain of Rubella virus that corresponds to AA₃₁₃ -AA₃₁₉ (SEQ ID NO: 7) of said protein of the M33 strain of Rubella virus as set forth in FIG. 1 (SEQ ID NO: 1);

(iv) the amino acid sequence of the Structural Polyprotein of a strain of Rubella virus that corresponds to AA₁₀₃ -AA₁₀₉ (SEQ ID NO: 10) of said protein of the Therien strain of Rubella virus as set forth in FIG. 2 (SEQ ID NO: 8);

(v) the amino acid sequence of the Structural Polyprotein of a strain of Rubella virus that corresponds to AA₉₀₀ -AA₉₆ of said protein of the Therien strain of Rubella virus as set forth in FIG. 2 (SEQ ID NO: 8);

(vi) the amino acid sequence of the Structural Polyprotein of a strain of Rubella virus that corresponds to AA₃₁₄ -AA₃₂₀ of said protein of the Therien strain of Rubella virus as set forth in FIG. 2 (SEQ ID NO: 8);

(vii) the amino acid sequence of the Gag Polyprotein of an isolate of the HIV-1 that corresponds to AA₁₄₅ -AA₁₅₁ (SEQ ID NO: 10) of the Gag polyprotein of the LV isolate of HIV-1 as set forth in FIG. 3 (SEQ ID NO:11);

(viii) the amino acid sequence of the Envelope Polyprotein Precursor of an isolate of the HIV-1 that corresponds to AA₆₅₅ to AA₆₆₁ (SEQ ID NO: 16) of the Envelope Polyprotein Precursor of the LAV-1a isolate of HIV-1 as set forth in FIG. 4 (SEQ ID NO: 14);

(ix) the amino acid sequence that corresponds to AA₉₉ -AA₁₀₅ (SEQ ID NO: 19) of the Lipoprotein E Precursor of Haemophilus influenzae as set forth in FIG. 5 (SEQ ID NO: 17);

(x) the amino acid sequence that corresponds to AA₁, to AA₅ (SEQ ID NO: 2) of the Opacity-Related Protein POPM3 of Neisseria meningitidis as set forth in FIG. 6 (SEQ ID NO: 20);

(xi) the amino acid sequence that corresponds to AA₄₂₃ to AA₄₂₉ (SEQ ID NO: 25) of the Pneumococcal Surface Protein A of Streptococcus pneumoniae as set forth in FIG. 7(SEQ ID NO: 23):

(xii) the amino acid sequence that corresponds to AA₁₅₁ -AA₁₅₇ (SEQ ID NO: 28) of the Protein P60 Precursor of Listeria monocytogenes as set forth in FIG. 8 (SEQ ID NO: 26);

(xiii) the amino acid sequence that corresponds to AA₁₈₁ -AA₁₈₇ (SEQ ID NO: 30) of the Protein P60 Precursor of Listeria monocytogenes as set forth in FIG. 8 (SEQ ID NO: 26);

(xiv) from the amino acid sequence of that corresponds to AA₂₆₉ -AA₂₅₅ (SEQ ID NO: 32) of the Protein P60 Precursor of Listeria monocytogenes as set forth in FIG. 8 (SEQ ID NO: 26);

(xv) from the amino acid sequence that corresponds to AA₂₉₂ -AA₂₉₈ (SEQ ID NO:34) of the Protein P60 Precursor of Listeria monocytogenes as set forth in FIG. 8 (SEQ ID NO: 26);

(xvi) from the amino acid sequence of a variant of the chemokine human Monocyte Chemoattractant Factor hMCP-1, that corresponds to AA₉₃ -AA₉₉ (SEQ ID NO: 37) of hMCP-1 as set forth in FIG. 9 (SEQ ID NO: 3);

(xvii) from the amino acid sequence of the chemokine hMCP-3, that corresponds to AA₆₁ -AA₆₇ SEQ ID NO: 40 of hMCP-3 as set forth in FIG. 10 (SEQ ID NO: 38); and

(xviii) from any amino acid sequence present within a protein that is homologous to members of the MRHAS family;

with said block maintaining the sequence in the N terminus to C terminus direction of the native amino acid sequence and analogue thereof, said analogues resulting from conservative substitutions in or modifications to the native amino acid sequence block;

a is selected from the group consisting of:

(i) an amino terminus;

(ii) one to eight amino acids taken as a block from and maintaining the sequence and N terminus to C terminus direction of that portion of the native amino acid sequence of the protein immediately N-terminal to said X or conservative substitutions in or modifications thereto; and

(iii) a substituent effective to facilitate coupling of the peptide to another moiety; and

b is selected from the group consisting of:

(i) a carboxy terminus;

(ii) one to eight amino acids taken as a block from and maintaining the sequence and N terminus to C terminus direction of that portion of the native amino acid sequence of the protein immediately C-terminal to said X or conservative substitutions in or modifications thereto; and

(iii) a substituent effective to facilitate coupling of the peptide to another moiety,

the improvement comprising the method of screening for bacterial and/or viral meningitis etiologic agents in one test.

Yet a further embodiment of the present invention is a method for analyzing a sample of a biological fluid with regard to the presence of anti-X antibodies therein, where X is selected from one or more members of the group comprising:

(i) Rubella virus;

(ii) HIV-1;

(iii) Hemophilus influenzae;

(iv) Nisteria meningitidis;

(v) Streptococcus pneumoniae;

(vi) Listeria monocytogenes, and

comprising the steps of:

A) providing a solid support having bound thereto a peptide selected from one or more members of the MRHAS family, or said peptide is selected from one or more members of the MRHAS family comprising a peptide having the formula

    a---X---b

wherein:

X is a sequence of at least 7 amino acids taken as a block selected from the group comprised in Table 5, and with said block maintaining the sequence in the N terminus to C terminus direction of the native amino acid sequence and analogue thereof, said analogues resulting from conservative substitutions in or modifications to the native amino acid sequence block; a is selected from the group consisting of:

(i) an amino terminus;

(ii) one to eight amino acids taken as a block from and maintaining the sequence and N terminus to C terminus direction of that portion of the native amino acid sequence of the protein immediately N-terminal to said X or conservative substitutions in or modifications thereto; and

(iii) a substituent effective to facilitate coupling of the peptide to another moiety; and

b is selected from the group consisting of:

(i) a carboxy terminus;

(ii) one to eight amino acids taken as a block from and maintaining the sequence and N terminus to C terminus direction of that portion of the native amino acid sequence of the protein immediately C-terminal to said X or conservative substitutions in or modifications thereto; and

(iii) a substituent effective to facilitate coupling of the peptide to another moiety,

B) contacting said solid support with said human sample to provide a sample-contacted support;

C) washing said sample-contacted support to provide a washed support; and

D) determining whether human antibodies are bound to said support.

Preparation and Use of Synthetic Peptides

Novel peptides are provided in the present invention which immunologically mimic protein epitopes encoded by infectious agents that cause meningitis and by monocyte-attracting chemokines. To accommodate variations among different infectious agents, adjustments for conservative substitutions, and selectionamong the alternatives where non-conservative substitutions are involved, may be made. There are many uses for these peptides which include, for example, use as: immunogens for a vaccine; blockers of MRHAS recognition sites on monocytes, interfering with the ability of meningitis etiologic agents to attract and infect monocytes and thereby block access of the infectious agent to the CNS; blockers of MRHAS recognition sites on monocytes involved in plaque build-up that occurs during atherosclerosis; and as antigens in diagnostic kits to detect antibodies in biological fluid as indication of infection by meningitis etiologic agents. Depending upon the nature of the protocol, the peptides may be conjugated to a carrier or other compounds, unlabeled or labeled, bound to a solid surface, or the like.

Embodiments of the present invention include peptides of interest derived from MRHAS family members listed in Table 1. (SEQ ID NOS 3, 5, 7, 10, 13, 16, 19, 22, 25, 28, 30, 32, and 34). Further embodiments include peptides of interest derived from MRHAS family members and their parent monocyte-attracting chemokines listed in Table 2(SEQ ID NO: 37 and 40). Other possible embodiments include MRHAS family members found on proteins listed in Table 3(SEQ ID NOS 2, 4, 6, 9, 12, 15, 18, 21, 24, 27, 29, 31, 33, 36 and 39).

The peptides of interest will include at least five, sometimes six, sometimes seven, sometimes eight, sometimes 15, sometimes 21, usually fewer than about 50 and preferably fewer than about 25 amino acids included within a sequence homologous to a member of the MRHAS family. It is desired that a given peptide be as small as possible while still maintaining all of the immunoreactivity or monocyte attracting activity or-the larger corresponding peptide. Furthermore, it may be desirable in some instances to join two or more oligopeptides which are non-overlapping to form a single peptide structure or to use them as individual peptides at the same time, which separately or together provide equivalent sensitivity to the parent.

A given peptide may be modified by introducing conservative or non-conservative substitutions in the peptide, usually fewer than 50 number percent, and more usually fewer than 30 number percent, more usually with fewer than 15 number percent of the amino acids being exchanged (Waterman, 1986, Nucleic Acids Res. 14:9095; Hitachi, HIBIO MacDNASIS Pro: DNA and Protein Sequence Analysis Software System Reference Manual, both incorporated in their entirety by reference). In those situations where amino acid regions are found to be polymorphic, it may be desirable to vary one or more particular amino acids to more effectively mimic the differing epitopes of the different meningitis etiologic infectious agents, or monocyte attracting chemokines.

It is important that it be understood that the polypeptide employed in the present invention need not be identical to any particular MRHAS family member, so long as the subject peptide is able to provide for immunological competition with proteins of at least one of the members of the MRHAS family and/or demonstrate monocyte recognition and/or attracting activity. Therefore, the subject peptide may be subject to various changes, such as substitutions, insertions, and deletions, either conservative or nonconservative, where such changes may provide for certain advantages in their use.

It is also important to point out that one, two, or more amino acids may be added to the termini of an oligopeptide or peptide to provide for ease of linking peptides one to another, for coupling to a support, or larger peptide and for reasons to be discussed subsequently, for modifying the physical or chemical properties of the peptide or oligopeptide, or the like.

In the present invention, the term amino acid is used to include, but not limited to, all natural occurring amino acids and all synthetic or non-natural amino acids such as homocysteine. The term `amino acids selected as a block` (or other similar statements) means a linear sequence of a set number of amino acids that taken together form a group. The term `antigenic determinant`0 means the structual component of an antigen molecule responsible for its specific interaction with antibody molecules elicited by the same or related antigen as defined by Dorland's Pocket Medical Dictionary 23ed. (Philadelphia: Saunders, 1982) at 198; Morris, ed. Academic Press Dictionary of Science and Technology (San Diego: Academic Press, 1992) which are both incorporated in their entirety by reference. The term `conservative substitution` means the substitution of one or more amino acids for another in which the antigenic determinant (including its secondary structure and hydropathic nature) of a given antigen is completely or partially conserved in spite of the substitution. The term `analogues of a peptide` means amino acid insertions, deletions, substitutions, and modifications of one or more sites in the peptide chain. The term `immunogenic` means the property that endows a substance with the capacity to provoke an immune response (Dorland, infra). The terms `corresponds` and `corresponding` refers to the native amino acids of those defined region of a given peptide sequence. Finally, amino acids such as cysteine, lysine, glutamic or aspartic acid, tyrosine, or the like may be introduced at the C- or N-terminus of a given peptide or oligopeptide to provide for a useful functionality for linking purposes. It will be appreciated by those skilled in the art that cysteine is particularly preferred to facilitate covalent coupling to other peptides or to form polymers by oxidation.

Moreover, a given peptide or oligopeptide sequence may differ from the natural sequence by the sequence being modified by terminal-NH₂ acylation (eg. by acetylation, or thioglycolic acid amidation, terminalcarbosy amidation, e.g., with ammonia or methylamine) to provide stability, increased hydrophobidity for Linking or binding to a support or other molecule, or for purposes of polymerization.

Of particular interest to the present invention is the use of the mercaptan group of cysteins or thioglycolic acids used for acylating terminal amino groups, or the like, for linking two of the peptides or oligopeptides or combinations thereof by a disulfide linkage or a longer linkage to form polymers that contain a number of MRHAS epitopes. Such polymers have the advantage of increased immunological reaction. Furthermore, where different peptides are used to make up the polymer, they possess the additional ability to induce antibodies that immunoreact with several antigenic determinants of the different meningitis etioligic agents.

In order to achieve the formation of antigenic polymers (ie. synthetic multimers), compounds may be utilized having bishaloacetyl groups, nitroarylhalides, or the like, where the reagents being specific for thio groups. Therefore, the link between two mercapto groups of the different peptides or oligopeptides may be a single bond or may be composed of a linking group of at least two, typically at least four, and not more than about 16, but usually not more than about 14 carbon atoms.

To prepare the novel peptides of the present invention, any of the conventional peptide production techniques may be employed. These techniques include synthesis, recombinant DNA technology and combinations thereof. The peptide may be synthesized in solution or on a solid support in accordance with conventional techniques. A variety of automatic synthesizers are commercially available and can be used in accordance with known protocols. For example, see Stewart & Young, 1984, Solid Phase Peptide Synthesis 2nd ed., Pierce Chemical Co.; Tam et al., 1983, J. Am Chem. Soc. 105:6442 which are both incoporated herein by reference. Recombinant DNA technology may be utilized where a synthetic gene may be prepared by employing single strands which code for the given MRHAS polypeptide or substantially complementary strands thereof, where the single strands overlap and can be brought together in an annealing medium so as to hybridize. The hybridized strands may then be ligated to form the complete gene, and, by choice of appropriate termini, the gene may be inserted into expression vectors, which are readily available today. For example, see Maniatis et al., 1982, Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory which is herein incorporated by reference. In the alternative, the region of the genome coding for the given MRHAS peptide may be cloned by conventional recombinant DNA techniques and expressed (See Maniatis, infra).

It is therefore, a preferred embodiment of this invention that there be a peptide having the formula a--X--b wherein, X is a sequence of at least 7 amino acids taken as a block selected from the group comprised in Table 5, with said block maintaining the sequence in the N terminus to C terminus direction of the native amino acid sequence and analogue thereof, said analogues resulting from conservative substitutions in or modifications to the native amino acid sequence block;

a is selected from the group consisting of:

(i) an amino terminus;

(ii) one to eight amino acids taken as a block from and maintaining the sequence and N terminus to C terminus direction of that portion of the native amino acid sequence of the protein immediately N-terminal to said X or conservative substitutions in or modifications thereto; and

(iii) a substituent effective to facilitate coupling of the peptide to another moiety; and

b is selected from the group consisting of:

(i) a carboxy terminus;

(ii) one to eight amino acids taken as a block from and maintaining the sequence and N terminus to C terminus direction of that portion of the native amino acid sequence of the protein immediately C-terminal to said X or conservative substitutions in or modifications thereto; and

(iii) a substituent effective to facilitate coupling of the peptide to another moiety.

Other features and advanteges of the present invention will become apparent from the following experimental descriptions, which describe the invention by way of example. The examples are offered by way of illustration and not by way of limitation.

EXAMPLE I

Generation and Characterization of Monoclonal Antibodies

Example I describes the method for the generation of hybridoma cell lines that produce monoclonal antibodies with a specificity for MRHAS. This method involves the use of purified Rubella virus as the immunogen. The protocols for the generation of the hybridima cell lines that produce the said monoclonal antibody and the characterization of the antibodies were as follows.

Rubella virus, strain M33, was obtained as the first passage after primary isolation. The RV strain was obtained from the laboratories of the National Institute of Health. Murine fibroblasts (L cells), used to generate stock virus, were maintained in monolayer cultures and were routinely propagated at 37° C. with minimal Eagle's medium (MEM) supplemented with 5% fetal calf serum (FCS, Grand Island Biological Company, GIBCO), 100 μg/ml streptomycin, and 100 IU/ml penicillin. Stock virus was routinely prepared by inoculating semiconfluent monolayers of L cells with RV at a multiplicity of infection (m.o.i.) of 0.01. After adsorption at 34° C. in a humid atmosphere containing 5% C02 for 1 hour, additional medium was added and the flask was incubated at 34° C for 6 days, at which time the culture supernatant was collected and frozen at -80° C.

Virus purification was accomplished as follows. L cell monolayers were infected at an m.o.i. of 0.01 and incubated at 34° C. for 6 days as described. The culture supernatants were collected and centrifuged at 3000×g for 20 min. All procedures were carried out a 4° C. unless otherwise stated. The supernatant obtained was recentrifugated at 100,000×g for 3 hours and the resulting pellet was resuspended in 0.2 ml TNE buffer (0.15 M NaC1, 50 Mm Tris-HCI, and 1 mM EDTA, pH 7.8). This sample was layered onto a 16 mi 25-45% discontinuous Renografin-60 (Reno M-60, Diatrisoate Meglumine, 60%, Squibb) gradient prepared with TNE buffer and centrifuged in an SW 27 Rotor at 55,000×g for 2 hours. The single, sharp band at the interface was collected, pelleted as described previously, resuspended in 0.5 ml TNM buffer (0.15 M NaCl, 50 mM Tris-HCI, 1 mM MgCl₂, pH 7.8), and layered on a 12 ml 30-45% continuous Renografin gradient prepared with TNM buffer. After centrifugation at 200,000×g for 3 hours, 0.5 ml fractions were collected. An aliquot was removed from each fraction for ELISA and infectivity tests (both described below). Appropriate fractions were then pooled, diluted with TNM buffer, and centrifuged at 100,000×g for 3 hours to remove the Renographin. Rubella antigen, prepared in this way, was used to immunize mice for the construction of hybridomas.

The ELISA was performed according to the procedure described by Volier in Rose & Freidman, eds., 1976, Manual of Clinical Immunology:506-512 which is incoporated herein by reference. Viral samples were diluted into coating buffer and duplicate 200 μl aliquots were adsorbed to microtiter plate wells (Cooke Laboratory Products, Dynatech Laboratories Inc., Alexandria, Va.). After coating, a predetermined 1/16 dilution of human anti-Rubella antiserum (H1 titer=1/128) was added to each well. Antibody binding was measured using a previously determined 1/2,000 dilution of rabbit antihuman IgG (Flow Laboratories) linked to alkaline phosphatase. The A400nm was determined after 30 minutes incubation at room temperature.

The infectivity test is a technique used to titer RV and was based on the ability of RV-infected cells to adsorb erythrocytes. It employs, in principle, the procedure incorporated herein by reference of Hotchin et al., 1960, Virology 10:275-280 for measuring the infectivity of noncytopathic viruses. Serial doubling dilutions of RV suspensions were used to infect confluent monolayers of L2 cells grown in tissue culture chamber slides (Lab Tek Products, Division of Miles Laboratories, Inc., Illinois). Two-chamber slides were used. Each chamber received a 50 μl aliquot of the appropriate RV dilution. Virus was allowed to adsorb for 1 hour at 34° C. and 2.5 ml of medium and 50 μl of a 20% suspension of heparinized sheep erythrocytes in Alserver's solution were added directly to each chamber. The slides were then incubated for 24 hours at 34° C. The chambers were removed and each slide was washed gently by immersion if pH 7.4 Dulbecco phosphate-buffered saline (PBS) at room temperature and examined microscopically for hemadsorbing cells. Uninfected control monolayers were treated in an identical fashion.

Mice were immunized using the following procedure. A Balb/c mouse was inoculated intraperitoneally (IP) with 250 μg of M.tuberculosis and 15 μg of purified RV suspended in 45% Renografin. Approximately 4 weeks later, 4 booster doses of 10 μg of virus each were given intravenously at day minus 5, minus 4, minus 3 and minus 2, prior to fusion. The final boost was accompanied by an additional injection of the same dose IP. Serum was taken from the immunized mouse throughout to monitor antibody production against RV proteins.

A Balb/c mouse was immunized as previously described and one day after the final booster doses of purified virus, the mouse was sacrificed and a suspension of spleen cells was prepared and fused with myeloma cells (P3X63Ag8) in a ratio of 5:1 using 50% polyethylene glycol according to the procedure described by Koprowski et al., 1977, Proc. Natl. Acad. Sci. 74:2985-2988 incorporated herein by reference. Cultures containing 1×105 cells in 100 μl were established in 96-2311 Linbro plastic plates (Flow Laboratories, McLean, Va., USA) where each well contained a feeder layer of 4×103 murine peritoneal exudate cells (macrophages). Colonies appeared in 2 to 3 weeks and culture medium in appropriate well were screened for anti-Rubella antibody in the ELISA employing infected and uninfected L cell lysates as antigen. Cells that were producing antibody were subcloned and retested.

ELISA screening of clones was performed according to the procedure described by Voller, infra, as previously described. Infected L cell monolayers were detached by scraping, sonicated and diluted in coating buffer to give a final protein concentration of 100 μg protein/100 μl of lysate. Each microwell was coated with 200 μl of lysate. After coating overnight at 4° C., 100 μl of each test supernatant was added. After a 90 minute incubation at 37° C., and washing, 100 μl of rabbit anti-mouse IgG, linked to alkaline phosphatase (Flow Laboratories) was added, and the plate was reincubated for one hour at 37° C. After addition of 100 μl of a 10% diethanolamine solution (pH 9.8), containing 1 mg/ml p-nitrophenylphosphate (Sigma), the plate was incubated for one hour at 37° C. and the A400nm was determined as before.

The immunoglobulin class of anti-Rubella virus antibodies produced by the positive clones was determined by testing the supernatant from such clones against affinity purified anti-mouse immunoglobulin (South Biotech), using the ELISA methods.

Polyacrylamide slab gel electrophoresis (PAGE) of Rubella virus proteins was performed according to Laemmli, 1970, Nature 227:680-685 incorporated herein by reference. RV polypeptides in sample buffer (0.062 M Tris-HCI, pH 6.8) containing 2% SDS, 1% (v/v) glycerol, 0.5% (w/v) bromophenol blue and 1% 2-mercaptoethanol were placed in a boiling water bath for 2 minutes prior to electrophoresis at 25 mA for 2 hours on a 10% discontinuous acrylamide slab gel system. Aliquots of 15 μl containing 5 μg of protein were applied to each gel lane. Protein standards used for gel calibration were as follows: bovine serum albumin (66200), ovalbumin (45,000), carbonic anhydrase (28,000), soybean trypsin inhibitor (20,100), and alpha-lactalbumin (14,200) (Bio-Rad). Gels were stained with silver according to the procedure described by Wray et al., 1981, AnalFt. Biochem. 118:197-203 incorporated herein by reference.

Rubella virus proteins separated by PAGE were transferred electrophoretically from the SDS-PAGE gel to nitrocellulose paper (Bio-Rad) by the method described by Towbin et al., 1979, Proc. Nat. Aced. Sci. 76:4350-4354 incorporated herein by reference. A constant current of 35 mA was applied to the gel-nitrocellulose paper sandwich for 1 hour, in an electroblot buffer of 25 mM Tris-HCI, 192 mM glycine and 20% (v/v) methanol at pH 8.3. The proteins transferred onto the blot were either stained with amido black or detected by enzyme immunoassay. The latter was performed by soaking the paper in PBS containing 1% milk for 30 minutes in order to clock non-specific protein binding sites. The paper was then

incubated with monoclonal antibody at 37° C. for 1 hour., washed 3 times with PBS followed by and hour incubation at 37° C. with peroxidase-conjugated goat anti-mouse immunoglobulin (Cappel, Cochranville, Pa.) diluted 1/1000 in PBS containing 3% BSA. After 3 additional washes, the blots were soaked in a solution of 0-dianisidine prepared as described by Towbin, infra.

Characterization of Mabs directed against RV 30,000 dalton protein

One fusion yielded 268 clones. After initial screening, 12 (4.5%) of the 266 clones were positive against infected cell lysates. The 12 clones were recloned and only 4 of these remained stable antibody producers. The 4 clones as listed in Table 6 were designed RV1-RV4 and further characterized according to Ig class and molecular weight of the antigen recognized.

                  TABLE 6     ______________________________________     Summary of Mab characteristics of 4 stable hybridoma     clones obtained                                          Molecular                                          weight of            Cell line                     Immuno-              antigen     Original            Desig-   globulin             recognized     done   nation   Class/subclass                                   A 410 nm                                          (Kd)     ______________________________________     101 B1 RV1      --            0.248  --     201 A5 RV2      --            0.126  --     6C6    RV3      --            0.241  --     1A1    RV4      --            0.174  --     ______________________________________

The first band to appear on immunoblotting was consistently the p30 core protein. However, a second band was observed at approximately 40,000 Kd and was clear after 30 minutes incubation. The larger 40 Kd protein has been designated E2 and has been shown to have a molecular weight of 35-38 Kd (vaccine strain and wild type 349). The E2 membrane protein is glycosylated and is detected in mature virions as a protein with a molecular weight of approximately 40,000-43,000 daltons. These results are summarized in FIG. 11.

The four hybridomas were isolated from a single fusion, but can be seen to be independent isolates from the differences observed in the immunoglobulin class determinations. In spite of their obvious differences, the clones were all directed against the same (cross-reacting) epitopes which appears to be on the RV core protein having a molecular weight of approximately 30,000.

A comparison of nucleotide sequences for the p30 core and p35-8 E1 sequences contained in the 24S subgenomic messenger RNA of RV (Zheng, 1989, infra) in Table 7 revealed that one core sequence SEQ ID NO: 3 was homologous with one E2 sequence SEQ ID NO: 7 as follows:

                  TABLE 7     ______________________________________     COMPARISON OF SEQUENCE HOMOLOGIES BETWEEN     p30 AND p38 IN THE RUBELLA VIRUS GENOME                 AMINO ACID     ORIGIN      POSITION     SEQUENCE     ______________________________________     RV (p30) core                 102          Q--P--Q--P--P--R--M     RV (E2) membrane                 313          P--P--Q--P--P--R--A     ______________________________________

In view of the core/outer membrane cross-reactivity of the RV monoclonal antibodies, it was certain that these antibodies would detect the presence of both p30 core and E2 membrane proteins, therby limiting their use in any diagnostic system which would attempt to define the status of RV infection in the CNS as permissive, or non-permissive, for growth.

However, the significance of the external placement of the internal core sequence in the membrane-associated E2 protein represents an important viral strategy as noted that amino acid changes in the E2 protein of several alpha-viruses have been found in Sindbis virus (Davis et al. , 1986, Proc. Natl. Acad. Sci. 83; 6771-6775), Ross River virus (Faragher et al., 1988, Virology 163:509-526) and Venezuelan equine encephalitis virus (Johnson et al., 2986, J. Gne. Virol. 67:1951-1960), to be implicated in the modulation of viral virulence.

EXAMPLE 2

The Use of RV1 Mab to Detect and Define Homologous Meningitis-Specific Antigenic sequences

In the course of RV1 Mab Characterization, it was observed that the RV1 Mab cross-reacted with bacterial antigens in N. meningitidis, S. Pneumoniae, H. Influenzae, L. monocytogenes as well as antigens in HIV-1. Immunoblots were performed as previously described using bacterial antigens and HIV-1 antigens and RV-1Mab.

Bacterial Strains and Culture Conditions

The bacterial strains were obtained from the American Type Culture Collection (ATCC), Washington, D.C. (Neisseria meningitidis and Streptococcus pneumoniae) and from the Caribbean Epidemiology Centre (CAREC), Port of Spain, Trinidad (Streptococcus pneumoniae). All strains were grown on chocolate agar overnight at 37° C. in an atmosphere containing 5% C02. Cultures were stored in brain heart infusion broth containing 20% glycerol at -7° C.

Antigert Preparation for PAGE and Immunoblotting

Antigens present in the outer membrane protein fraction of Neisseria meningitidis were prepared using lithium chloride as previously described by Johnston et al., 1976, J. Exp. Med. 143: 741-758 incorporated herein by reference. Whole cells were suspended in lithium chloride buffer (200 mM lithium chloride, 100 mM lithium acetate, 10 mM EDTA, pH 6.0), transferred to a 250 ml erleluneyer flask containing 3-5 mm glass beads and shaken at 300 rpm in a G24 Environmental incubator shaker for 2 hours at 45° C. The suspension was centrifuged at 8,000 rpm for 20 minutes using a Sorvall SS034 fixed angle rotor with R max=10.70 cm. Collected supernatant was transferred to a rigid wall polycarbonate tube and centrifuged at 35,000 rpm for 2 hr at 10° C. using a 50.2 Beckman rotor. The supernatant was discarded and pellet resuspended in 1 ml of phosphate buffered saline (PBS). The protein content was determined by the Lowry method.

Sonicated antigen preparations of S. pneumoniae and H. influenzae were prepared using the following procedure. Approximately 10¹¹ bacteria were suspended in 5 ml PBS and heat-killed for 20 min at 56° C. Using a Branso 350 Sonifier Cell disruper (Branson Cleaning Equipment Co.) cells were sonicated 3 times, with a 50% pulse setting, for 5 minutes each time. The sample was kept at 4° C. with ice throughout. The suspensions were then centrifuged for 20 min at 25,000 rpm, using a Beckman 70 Ti.1 rotor at 10° C. The protein concentration of the resulting supernatant was determined using the Lowrey protein assay.

HIV-1 antigen was purchased from ABI (Advanced Biotechnologies, Inc., Columbia, Md). Antigen was contained in viral lysate with specifications given in catalog number 10-119-000 with Lot number 54-040 containing a particle count of 1.09×10¹⁰ vp/ml active virus. The preparation was treated with Triton X-100 added to a final concentration of 1%, and heated to 56° C. for one hour with mixing. The final protein concentration of lysate was 0.78 mg/ml. Each lane for PAGE contained 10 μg of antigen.

PAGE and Immunoblotting procedures.

PAGE was carried out as previously described using 15 μl samples of bacterial antigen, containing 5 μg protein per well. Immunoblots were performed on the transferred antigens using RV1 Mab in tissue culture supernatants as previously described.

The results of immunoblots of bacterial antigen using RV1 Mab are contained in FIG. 12. The RIV Mab clearly detected cross-reacting epitopes in N. Meningitidis, influenzae, S. pneumoniae and eliminated all of these bands, indicating that the antigens detected with the RV Mab are protein in nature. Control Streptococcus A and M.tuberculosis (p60) antigen preparations were negative using the RV1 Mab.

The results of immunoblots of HIV antigens using RV1 Mab are contained in FIG. 13. The RV1 Mab clearly detected two membrane protein antigens indicating that HIV employs a strategy identical to that of RV which places a portion of the inner core protein on the outside of the virion.

Since the likely sequences of the corresponding RV1 Mab antigens are QPQPPRM SEQ ID NO: 3 and PPQPPCA SEQ ID NO: 7 in the core and E2 proteins, respectively, a search was undertaken to find similar, cross

reacting sequences in the available bacterial and HIV sequences, with results the data presented in TABLE 4.

FIG. 12 illustrates a cross-reactivity, with the RV1 Mab detecting a major band of apporximately 26-28,000 daltons and 2 minor bands at approximately 45,000 daltons. An outer membrane protein with a molecular weight of about 28,000, expressed on the cell surface, and existing as a lipoprotein in association with the outer membrane-cell wall complex of H. influenzae has been identified and designated Protein E. It is capable of eliciting a bactericidal immune response against non-typable Influenzae and is highly conserved among H.influenzae strains. Protein E has been sequence (Green and Z lotnick, infra ) and the sequences listed in Table 4 (SEQ ID NOS 3, 5, 7, 10, 13, 16, 19, 22, 25, 28, 30, 32, 34, 37 and 40) are closely homologous to the membrane and core sequences of RV shown in that table.

FIG. 12 also illustrates that the RV1 Mab detected one band at approximately 60,000 daltons with L.monocytogenes. All virulent L.monocytogenes stains secrete as SH-activated cytolysin called listeriolysin (Kuhn & Goebel, 1988, Infect. Immun. 56:79-82). This protein, termed p60, is an essential virulence factor as nonhemolytic mutants have reduced rates of survival in the mouse infection model (Galliard, et al., 1986, Infect. Immun. 52:50-55) and in mouse peritoneal macrophages (Kuhn & Goebel, infra). The sequence of the p60 has been determined (Kohler, et al., 1990, Infec. Immun. 58:1943-1950) and the sequences identified at the positions listed in Table 4 (SEQ ID NOS 3, 5, 7, 10, 13, 16, 19, 22, 25, 28, 30, 32, 34, 37 and 40);are closely homologous to the RV core and membrane sequence.

Finally, FIG. 13 illustrates that the RV Mab detected two bands at approximately 24,000 (p24) and 61,000 (p61) daltons. The p24 has been shown to be a major core protein and p61 a transmembrane protein in the HIV virion, and the complete nuceotide sequence of the HIV1 genome is available (Ratner et al., 1985, Nature 313:277-280). A number of septapeptide sequences were identified which are closely homologous to the RV core and membrane sequences, and these sequences are listed in Table 4 (SEQ ID NOS 3, 5, 7, 10, 13, 16, 19, 22, 25, 28, 30, 32, 34, 37 and 40).

    __________________________________________________________________________     SEQUENCE LISTING     (1) GENERAL INFORMATION:     (iii) NUMBER OF SEQUENCES: 40     (2) INFORMATION FOR SEQ ID NO:1:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 992 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:     MetAlaSerThrThrProIleThrMetGluAspLeuGlnLysAlaLeu     151015     GluAlaGlnSerArgAlaLeuArgAlaGlyLeuAlaAlaGlyAlaSer     202530     GlnSerArgArgProArgProProArgHisAlaArgLeuGlnHisLeu     354045     ProGluMetThrProAlaValThrProGluGlyProAlaProProArg     505560     ThrGlyAlaTrpGlnArgLysAspTrpSerArgAlaProProProPro     65707580     GluGluArgGlnGluSerArgSerGlnThrProAlaProLysProSer     859095     ArgAlaProProGlnGlnProGlnProProArgMetGlnThrGlyArg     100105110     GlyGlySerAlaProArgProGluLeuGlyProProThrAsnProPhe     115120125     GlnAlaAlaValAlaArgGlyLeuArgProProLeuHisAspProAsp     130135140     ThrGluAlaProThrGluAlaCysValThrSerTrpLeuTrpSerGlu     145150155160     GlyGluGlyAlaValPheTyrArgValAspLeuHisPheIleAsnLeu     165170175     GlyThrProProLeuAspGluAspGlyArgTrpAspProAlaLeuMet     180185190     TyrAsnProCysGlyProGluProProAlaHisValValArgAlaTyr     195200205     AsnGlnProAlaGlyAspValArgGlyValTrpGlyLysGlyGluArg     210215220     ThrTyrAlaGluGlnAspPheArgValGlyGlyThrArgTrpHisArg     225230235240     LeuLeuArgMetProValArgGlyLeuAspGlyAspThrAlaProLeu     245250255     ProProHisThrThrGluArgIleGluThrArgSerAlaArgHisPro     260265270     TrpArgIleArgPheGlyAlaProGlnAlaPheLeuAlaGlyLeuLeu     275280285     LeuAlaAlaValAlaValGlyThrAlaArgAlaGlyLeuGlnProArg     290295300     AlaAspMetAlaAlaProProMetProProGlnProProArgAlaHis     305310315320     GlyGlnHisTyrGlyHisHisHisHisGlnLeuProPheLeuGlyHis     325330335     AspGlyHisHisGlyGlyThrLeuArgValGlyGlnHisHisArgAsn     340345350     AlaSerAspValLeuProGlyHisTrpLeuGlnGlyGlyTrpGlyCys     355360365     TyrAsnLeuSerAspTrpHisGlnGlyThrHisValCysHisThrLys     370375380     HisMetAspPheTrpCysValGluHisAspArgProProProAlaThr     385390395400     ProThrSerLeuThrThrAlaAlaAsnTyrIleAlaAlaAlaThrPro     405410415     AlaThrAlaProProProCysHisAlaGlyLeuAsnAspSerCysGly     420425430     GlyPheLeuSerGlyCysGlyProMetArgLeuProThrAlaLeuThr     435440445     ProGlyAlaValGlyAspLeuArgAlaValHisHisArgProValPro     450455460     AlaTyrProValCysCysAlaMetArgTrpGlyLeuProProTrpGlu     465470475480     LeuValIleLeuThrAlaArgProGluAspGlyTrpThrCysArgGly     485490495     ValProAlaHisProGlyThrArgCysProGluLeuValSerProMet     500505510     GlyArgAlaThrCysSerProAlaSerAlaLeuTrpLeuAlaThrAla     515520525     AsnAlaLeuSerLeuAspHisAlaPheAlaAlaPheValLeuLeuVal     530535540     ProTrpValLeuIlePheMetValCysArgArgAlaCysArgArgPro     545550555560     AlaProProProProSerProGlnSerSerCysArgGlyThrThrPro     565570575     ProAlaTyrGlyGluGluAlaPheThrTyrLeuCysThrAlaProGly     580585590     CysAlaThrGlnThrProValProValArgLeuAlaGlyValGlyPhe     595600605     GluSerLysIleValAspGlyGlyCysPheAlaProTrpAspLeuGlu     610615620     AlaThrGlyAlaCysIleCysGluIleProThrAspValSerCysGlu     625630635640     GlyLeuGlyAlaTrpValProThrAlaProCysAlaArgIleTrpAsn     645650655     GlyThrGlnArgAlaCysThrPheTrpAlaValAsnAlaTyrSerSer     660665670     GlyGlyTyrAlaGlnLeuAlaSerTyrPheAsnProGlyGlySerTyr     675680685     TyrLysGlnTyrHisProThrAlaCysGluValGluProAlaPheGly     690695700     HisSerAspAlaAlaCysTrpGlyPheProThrAspThrValMetSer     705710715720     ValPheAlaLeuAlaSerTyrValGlnHisProHisLysThrValArg     725730735     ValLysPheHisThrGluThrArgThrValTrpGlnLeuSerValAla     740745750     GlyValSerCysAsnValThrThrGluHisProPheCysAsnThrPro     755760765     HisGlyGlnLeuGluValGlnValProProAspProGlyAspLeuVal     770775780     GluTyrIleMetAsnTyrThrGlyAsnGlnGlnSerArgTrpGlyLeu     785790795800     GlySerProAsnCysHisGlyProAspTrpAlaSerProValCysGln     805810815     ArgHisSerProAspCysSerArgLeuValGlyAlaThrProGluArg     820825830     ProArgLeuArgLeuValAspAlaAspAspProLeuLeuArgThrAla     835840845     ProGlyProGlyGluValTrpValThrProValIleGlySerGlnAla     850855860     ArgLysCysGlyLeuHisIleArgAlaGlyProTyrGlyHisAlaThr     865870875880     ValGluMetProGluTrpIleHisAlaHisThrThrSerAspProTrp     885890895     HisProProGlyProLeuGlyLeuLysPheLysThrValArgProVal     900905910     AlaLeuProArgAlaLeuAlaProProArgAsnValArgValThrGly     915920925     CysTyrGlnCysGlyThrProAlaLeuValGluGlyLeuAlaProGly     930935940     GlyGlyAsnCysHisLeuThrValAsnGlyGluAspValGlyAlaPhe     945950955960     ProProGlyLysPheValThrAlaAlaLeuLeuAsnThrProProPro     965970975     TyrGlnValSerCysGlyGlyGluSerAspArgAlaSerAlaGlyHis     980985990     (2) INFORMATION FOR SEQ ID NO:2:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 21 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:     ProSerArgAlaProProGlnGlnProGlnProProArgMetGlnThr     151015     GlyArgGlyGlySer     20     (2) INFORMATION FOR SEQ ID NO:3:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 7 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:     GlnProGlnProProArgMet     15     (2) INFORMATION FOR SEQ ID NO:4:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 21 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:     GluArgGlnGluSerArgSerGlnThrProAlaProLysProSerArg     151015     AlaProProGlnGln     20     (2) INFORMATION FOR SEQ ID NO:5:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 7 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5:     GlnThrProAlaProLysPro     15     (2) INFORMATION FOR SEQ ID NO:6:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 21 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6:     AspMetAlaAlaProProMetProProGlnProProArgAlaHisGly     151015     GlnHisTyrGlyHis     20     (2) INFORMATION FOR SEQ ID NO:7:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 7 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:7:     ProProGlnProProArgAla     15     (2) INFORMATION FOR SEQ ID NO:8:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 1063 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:8:     MetAlaSerThrThrProIleThrMetGluAspLeuGlnLysAlaLeu     151015     GluAlaGlnSerArgAlaLeuArgAlaGluLeuAlaAlaGlyAlaSer     202530     GlnSerArgArgProArgProProArgGlnArgAspSerSerThrSer     354045     GlyAspAspSerGlyArgAspSerGlyGlyProArgArgArgArgGly     505560     AsnArgGlyArgGlyGlnArgArgAspTrpSerArgAlaProProPro     65707580     ProGluGluArgGlnGluSerArgSerGlnThrProAlaProLysPro     859095     SerArgAlaProProGlnGlnProGlnProProArgMetGlnThrGly     100105110     ArgGlyGlySerAlaProArgProGluLeuGlyProProThrAsnPro     115120125     PheGlnAlaAlaValAlaArgGlyLeuArgProProLeuHisAspPro     130135140     AspThrGluAlaProThrGluAlaCysValThrSerTrpLeuTrpSer     145150155160     GluGlyGlnGlyAlaValPheTyrArgValAspLeuHisPheThrAsn     165170175     LeuGlyThrProProLeuAspGluAspGlyArgTrpAspProAlaLeu     180185190     MetTyrAsnProCysGlyProGluProProAlaHisValValArgAla     195200205     TyrAsnGlnProAlaGlyAspValArgGlyValTrpGlyLysGlyGlu     210215220     ArgThrTyrAlaGluGlnAspPheArgValGlyGlyThrArgTrpHis     225230235240     ArgLeuLeuArgMetProValArgGlyLeuAspGlyAspSerAlaPro     245250255     LeuProProHisThrThrGluArgIleGluThrArgSerAlaArgHis     260265270     ProTrpArgIleArgPheGlyAlaProGlnAlaPheLeuAlaGlyLeu     275280285     LeuLeuAlaThrValAlaValGlyThrAlaArgAlaGlyLeuGlnPro     290295300     ArgAlaAspMetAlaAlaProProThrLeuProGlnProProCysAla     305310315320     HisGlyGlnHisTyrGlyHisHisHisHisGlnLeuProPheLeuGly     325330335     HisAspGlyHisHisGlyGlyThrLeuArgValGlyGlnHisTyrArg     340345350     AsnAlaSerAspValLeuProGlyHisTrpLeuGlnGlyGlyTrpGly     355360365     CysTyrAsnLeuSerAspTrpHisGlnGlyThrHisValCysHisThr     370375380     LysHisMetAspPheTrpCysValGluHisAlaArgProProProAla     385390395400     ThrProThrProLeuThrThrAlaAlaAsnSerThrThrAlaAlaThr     405410415     ProAlaThrAlaProAlaProCysHisAlaGlyLeuAsnAspSerCys     420425430     GlyGlyPheLeuSerGlyCysGlyProMetArgLeuArgHisGlyAla     435440445     AspThrArgCysGlyArgLeuIleCysGlyLeuSerThrThrAlaGln     450455460     TyrProProThrArgPheGlyCysAlaMetArgTrpGlyLeuProPro     465470475480     TrpGluLeuValValLeuThrAlaArgProGluAspGlyTrpThrCys     485490495     ArgGlyValProAlaHisProGlyAlaArgCysProGluLeuValSer     500505510     ProMetGlyArgAlaThrCysSerProAlaSerAlaLeuTrpLeuAla     515520525     ThrAlaAsnAlaLeuSerLeuAspHisAlaLeuAlaAlaPheValLeu     530535540     SerValProTrpValLeuIlePheMetValCysArgArgAlaCysArg     545550555560     ArgArgGlyAlaAlaAlaAlaLeuThrAlaValValLeuGlnGlyTyr     565570575     AsnProProAlaTyrGlyGluGluAlaPheThrTyrLeuCysThrAla     580585590     ProGlyCysAlaThrGlnAlaProValProValArgLeuAlaGlyVal     595600605     ArgPheGluSerLysIleValAspGlyGlyCysPheAlaProTrpAsp     610615620     LeuGluAlaThrGlyAlaCysIleCysGluIleProThrAspValSer     625630635640     CysGluGlyLeuGlyAlaTrpValProAlaAlaProCysAlaArgIle     645650655     TrpAsnGlyThrGlnArgAlaCysThrPheTrpAlaValAsnAlaTyr     660665670     SerSerGlyGlyTyrAlaGlnLeuAlaSerTyrPheAsnProGlyGly     675680685     SerTyrTyrLysGlnTyrHisProThrAlaCysGluValGluProAla     690695700     PheGlyHisSerAspAlaAlaCysTrpGlyPheProThrAspThrVal     705710715720     MetSerValPheAlaLeuAlaSerTyrValGlnHisProHisLysThr     725730735     ValArgValLysPheHisThrGluThrArgThrValTrpGlnLeuSer     740745750     ValAlaGlyValSerCysAsnValThrThrGluHisProPheCysAsn     755760765     ThrProHisGlyGlnLeuGluValGlnValProProAspProGlyAsp     770775780     LeuValGluTyrIleMetAsnTyrThrGlyAsnGlnGlnSerArgTrp     785790795800     GlyLeuGlySerProAsnCysHisGlyProAspTrpAlaSerProVal     805810815     CysGlnArgHisSerProAspCysSerArgLeuValGlyAlaThrPro     820825830     GluArgProArgLeuArgLeuValAspAlaAspAspProLeuLeuArg     835840845     ThrAlaProGlyProGlyGluValTrpValThrProValIleGlySer     850855860     GlnAlaArgLysCysGlyLeuHisIleArgAlaGlyProTyrGlyHis     865870875880     AlaThrValGluMetProGluTrpIleHisAlaHisThrThrSerAsp     885890895     ProTrpHisProProGlyProLeuGlyLeuLysPheLysThrValArg     900905910     ProValAlaLeuProArgThrLeuAlaProProArgAsnValArgVal     915920925     ThrGlyCysTyrGlnCysGlyThrProAlaLeuValGluGlyLeuAla     930935940     ProGlyGlyGlyAsnCysHisLeuThrValAsnGlyGluAspValGly     945950955960     AlaValProProGlyLysPheValThrAlaAlaLeuLeuAsnThrPro     965970975     ProProTyrGlnValSerCysGlyGlyGluSerAspArgAlaSerAla     980985990     ArgValIleAspProAlaAlaGlnSerPheThrGlyValValTyrGly     99510001005     ThrHisThrThrAlaValSerGluThrArgGlnThrTrpAlaGluTrp     101010151020     AlaAlaAlaHisTrpTrpGlnLeuThrLeuGlyAlaThrCysAlaLeu     1025103010351040     ProLeuAlaGlyLeuLeuAlaCysCysAlaLysCysLeuTyrTyrLeu     104510501055     ArgGlyAlaIleAlaProArg     1060     (2) INFORMATION FOR SEQ ID NO:9:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 21 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:9:     AspMetAlaAlaProProThrLeuProGlnProProArgAlaHisGly     151015     GlnHisTyrGlyHis     20     (2) INFORMATION FOR SEQ ID NO:10:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 7 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:10:     LeuProGlnProProCysAla     15     (2) INFORMATION FOR SEQ ID NO:11:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 478 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:11:     MetGlyAlaArgAlaSerValLeuSerGlyGlyGluLeuAspArgTrp     151015     GluLysIleArgLeuArgProGlyGlyLysLysLysTyrLysLeuLys     202530     HisIleValTrpAlaSerArgGluLeuGluArgPheAlaValAsnPro     354045     GlyLeuLeuGluThrSerGluGlyCysArgGlnIleLeuGlyGlnLeu     505560     GlnProSerLeuGlnThrGlySerGluGluLeuArgSerLeuTyrAsn     65707580     ThrValAlaThrLeuTyrCysValHisGlnArgIleGluIleLysAsp     859095     ThrLysGluAlaLeuAspLysIleGluGluGluGlnAsnLysSerLys     100105110     LysLysAlaGlnGlnAlaAlaAlaAspThrGlyHisSerSerGlnVal     115120125     SerGlnAsnTyrProIleValGlnAsnIleGlnGlyGlnMetValHis     130135140     GlnAlaIleSerProArgThrLeuAsnAlaTrpValLysValValGlu     145150155160     GluLysAlaPheSerProGluValIleProMetPheSerAlaLeuSer     165170175     GluGlyAlaThrProGlnAspLeuAsnThrMetLeuAsnThrValGly     180185190     GlyHisGlnAlaAlaMetGlnMetLeuLysGluThrIleAsnGluGlu     195200205     AlaAlaGluTrpAspArgValHisProValHisAlaGlyProIleAla     210215220     ProGlyGlnMetArgGluProArgGlySerAspIleAlaGlyThrThr     225230235240     SerThrLeuGlnGluGlnIleGlyTrpMetThrAsnAsnProProIle     245250255     ProValGlyGluIleTyrLysArgTrpIleIleLeuGlyLeuAsnLys     260265270     IleValArgMetTyrSerProThrSerIleLeuAspIleArgGlnGly     275280285     ProLysGluProPheArgAspTyrValAspArgPheTyrLysThrLeu     290295300     ArgAlaGluGlnAlaSerGlnGluValLysAsnTrpMetThrGluThr     305310315320     LeuLeuValGlnAsnAlaAsnProAspCysLysThrIleLeuLysAla     325330335     LeuGlyProAlaAlaThrLeuGluGluMetMetThrAlaCysGlnGly     340345350     ValGlyGlyProGlyHisLysAlaArgValLeuAlaGluAlaMetSer     355360365     GlnValThrAsnThrAlaThrIleMetMetGlnArgGlyAsnPheArg     370375380     AsnGlnArgLysMetValLysCysPheAsnCysGlyLysGluGlyHis     385390395400     ThrAlaArgAsnCysArgAlaProArgLysLysGlyCysTrpLysCys     405410415     GlyLysGluGlyHisGlnMetLysAspCysThrGluArgGlnAlaAsn     420425430     PheLeuGlyLysIleCysLeuProThrArgGluGlyGlnGlyIlePhe     435440445     PheArgAlaAspGlnSerGlnGlnProHisHisPhePheArgAlaAsp     450455460     GlnSerGlnGlnProHisGlnLysArgAlaSerGlyLeuGly     465470475     (2) INFORMATION FOR SEQ ID NO:12:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 21 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:12:     IleGlnGlyGlnMetValHisGlnAlaIleSerProArgThrLeuAsn     151015     AlaTrpValLysVal     20     (2) INFORMATION FOR SEQ ID NO:13:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 7 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:13:     GlnAlaIleSerProArgThr     15     (2) INFORMATION FOR SEQ ID NO:14:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 861 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:14:     MetArgValLysGluLysTyrGlnHisLeuTrpArgTrpGlyTrpLys     151015     TrpGlyThrMetLeuLeuGlyIleLeuMetIleCysSerAlaThrGlu     202530     LysLeuTrpValThrValTyrTyrGlyValProValTrpLysGluAla     354045     ThrThrThrLeuPheCysAlaSerAspAlaLysAlaTyrAspThrGlu     505560     ValHisAsnValTrpAlaThrHisAlaCysValProThrAspProAsn     65707580     ProGlnGluValValLeuValAsnValThrGluAsnPheAsnMetTrp     859095     LysAsnAspMetValGluGlnMetHisGluAspIleIleSerLeuTrp     100105110     AspGlnSerLeuLysProCysValLysLeuThrProLeuCysValSer     115120125     LeuLysCysThrAspLeuGlyAsnAlaThrAsnThrAsnSerSerAsn     130135140     ThrAsnSerSerSerGlyGluMetMetMetGluLysGlyGluIleLys     145150155160     AsnCysSerPheAsnIleSerThrSerIleArgGlyLysValGlnLys     165170175     GluTyrAlaPhePheTyrLysLeuAspIleIleProIleAspAsnAsp     180185190     ThrThrSerTyrThrLeuThrSerCysAsnThrSerValIleThrGln     195200205     AlaCysProLysValSerPheGluProIleProIleHisTyrCysAla     210215220     ProAlaGlyPheAlaIleLeuLysCysAsnAsnLysThrPheAsnGly     225230235240     ThrGlyProCysThrAsnValSerThrValGlnCysThrHisGlyIle     245250255     ArgProValValSerThrGlnLeuLeuLeuAsnGlySerLeuAlaGlu     260265270     GluGluValValIleArgSerAlaAsnPheThrAspAsnAlaLysThr     275280285     IleIleValGlnLeuAsnGlnSerValGluIleAsnCysThrArgPro     290295300     AsnAsnAsnThrArgLysSerIleArgIleGlnArgGlyProGlyArg     305310315320     AlaPheValThrIleGlyLysIleGlyAsnMetArgGlnAlaHisCys     325330335     AsnIleSerArgAlaLysTrpAsnAlaThrLeuLysGlnIleAlaSer     340345350     LysLeuArgGluGlnPheGlyAsnAsnLysThrIleIlePheLysGln     355360365     SerSerGlyGlyAspProGluIleValThrHisSerPheAsnCysGly     370375380     GlyGluPhePheTyrCysAsnSerThrGlnLeuPheAsnSerThrTrp     385390395400     PheAsnSerThrTrpSerThrGluGlySerAsnAsnThrGluGlySer     405410415     AspThrIleThrLeuProCysArgIleLysGlnPheIleAsnMetTrp     420425430     GlnGluValGlyLysAlaMetTyrAlaProProIleSerGlyGlnIle     435440445     ArgCysSerSerAsnIleThrGlyLeuLeuLeuThrArgAspGlyGly     450455460     AsnAsnAsnAsnGlySerGluIlePheArgProGlyGlyGlyAspMet     465470475480     ArgAspAsnTrpArgSerGluLeuTyrLysTyrLysValValLysIle     485490495     GluProLeuGlyValAlaProThrLysAlaLysArgArgValValGln     500505510     ArgGluLysArgAlaValGlyIleGlyAlaLeuPheLeuGlyPheLeu     515520525     GlyAlaAlaGlySerThrMetGlyAlaArgSerMetThrLeuThrVal     530535540     GlnAlaArgGlnLeuLeuSerGlyIleValGlnGlnGlnAsnAsnLeu     545550555560     LeuArgAlaIleGluAlaGlnGlnHisLeuLeuGlnLeuThrValTrp     565570575     GlyIleLysGlnLeuGlnAlaArgIleLeuAlaValGluArgTyrLeu     580585590     LysAspGlnGlnLeuLeuGlyIleTrpGlyCysSerGlyLysLeuIle     595600605     CysThrThrAlaValProTrpAsnAlaSerTrpSerAsnLysSerLeu     610615620     GluGlnIleTrpAsnAsnMetThrTrpMetGluTrpAspArgGluIle     625630635640     AsnAsnTyrThrSerLeuIleHisSerLeuIleGluGluSerGlnAsn     645650655     GlnGlnGluLysAsnGluGlnGluLeuLeuGluLeuAspLysTrpAla     660665670     SerLeuTrpAsnTrpPheAsnIleThrAsnTrpLeuTrpTyrIleLys     675680685     IlePheIleMetIleValGlyGlyLeuValGlyLeuArgIleValPhe     690695700     AlaValLeuSerIleValAsnArgValArgGlnGlyTyrSerProLeu     705710715720     SerPheGlnThrHisLeuProThrProArgGlyProAspArgProGlu     725730735     GlyIleGluGluGluGlyGlyGluArgAspArgAspArgSerIleArg     740745750     LeuValAsnGlySerLeuAlaLeuIleTrpAspAspLeuArgSerLeu     755760765     CysLeuPheSerTyrHisArgLeuArgAspLeuLeuLeuIleValThr     770775780     ArgIleValGluLeuLeuGlyArgArgGlyTrpGluAlaLeuLysTyr     785790795800     TrpTrpAsnLeuLeuGlnTyrTrpSerGlnGluLeuLysAsnSerAla     805810815     ValSerLeuLeuAsnAlaThrAlaIleAlaValAlaGluGlyThrAsp     820825830     ArgValIleGluValValGlnGlyAlaCysArgAlaIleArgHisIle     835840845     ProArgArgIleArgGlnGlyLeuGluArgIleLeuLeu     850855860     (2) INFORMATION FOR SEQ ID NO:15:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 21 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:15:     HisSerLeuIleGluGluSerGlnAsnGlnGlnGluLysAsnGluGln     151015     GluLeuLeuGluLeu     20     (2) INFORMATION FOR SEQ ID NO:16:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 7 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:16:     GlnAsnGlnGlnGluLysAsn     15     (2) INFORMATION FOR SEQ ID NO:17:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 274 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:17:     MetLysThrThrLeuLysMetThrAlaLeuAlaAlaLeuSerAlaPhe     151015     ValLeuAlaGlyCysGlySerHisGlnMetLysSerGluGluHisAla     202530     AsnMetGlnLeuGlnGlnGlnAlaValLeuGlyLeuAsnTrpMetGln     354045     AspSerGlyGluTyrLysAlaLeuAlaTyrGlnAlaTyrAsnAlaAla     505560     LysValAlaPheAspHisAlaLysValAlaLysGlyLysLysLysAla     65707580     ValValAlaAspLeuAspGluThrMetLeuAspAsnSerProTyrAla     859095     GlyTrpGlnValGlnAsnAsnLysProPheAspGlyLysAspTrpThr     100105110     ArgTrpValAspAlaArgGlnSerArgAlaValProGlyAlaValGlu     115120125     PheAsnAsnTyrValAsnSerHisAsnGlyLysValPheTyrValThr     130135140     AsnArgLysAspSerThrGluLysSerGlyThrIleAspAspMetLys     145150155160     ArgLeuGlyPheAsnGlyValGluGluSerAlaPheTyrLeuLysLys     165170175     AspLysSerAlaLysAlaAlaArgPheAlaGluIleGluLysGlnGly     180185190     TyrGluIleValLeuTyrValGlyAspAsnLeuAspAspPheGlyAsn     195200205     ThrValTyrGlyLysLeuAsnAlaAspArgArgAlaPheValAspGln     210215220     AsnGlnGlyLysPheGlyLysThrPheIleMetLeuProAsnAlaAsn     225230235240     TyrGlyGlyTrpGluGlyGlyLeuAlaGluGlyTyrPheLysLysAsp     245250255     ThrGlnGlyGlnIleLysAlaArgLeuAspAlaValGlnAlaTrpAsp     260265270     GlyLys     (2) INFORMATION FOR SEQ ID NO:18:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 21 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:18:     AsnSerProTyrAlaGlyTrpGlnValGlnAsnAsnLysProPheAsp     151015     GlyLysAspTrpThr     20     (2) INFORMATION FOR SEQ ID NO:19:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 7 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:19:     GlnValGlnAsnAsnLysPro     15     (2) INFORMATION FOR SEQ ID NO:20:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 170 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:20:     IleGlnProProLysAsnLeuLeuPheSerSerLeuLeuPheSerSer     151015     LeuLeuPheSerSerAlaAlaGlnAlaAlaSerGluAspArgArgSer     202530     ProTyrTyrValGlnAlaAspLeuAlaTyrAlaAlaGluArgIleThr     354045     HisAspTyrProGlnAlaThrGlyAlaAsnAsnThrSerThrValSer     505560     AspTyrPheArgAsnIleArgAlaHisSerIleHisProArgValSer     65707580     ValGlyTyrAspPheGlyGlyTrpArgIleAlaAlaAspTyrAlaSer     859095     TyrArgLysTrpAsnAsnAsnLysTyrSerValAsnThrLysGluLeu     100105110     GluAsnLysHisAsnAsnLysLysAspLeuLysThrGluAsnGlnGlu     115120125     AsnGlyThrPheHisAlaAlaSerSerLeuGlyLeuSerAlaIleTyr     130135140     AspPheLysLeuLysGlyLysPheLysProTyrIleGlyAlaArgVal     145150155160     AlaTyrGlyHisValArgHisSerIleAsp     165170     (2) INFORMATION FOR SEQ ID NO:21:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 13 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:21:     IleGlnProProLysAsnLeuLeuPheSerSerLeuLeu     1510     (2) INFORMATION FOR SEQ ID NO:22:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 6 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:22:     IleGlnProProLysAsn     15     (2) INFORMATION FOR SEQ ID NO:23:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 695 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:23:     LysLeuMetIleXaaLysPheValThrLysMetXaaTyrLysThrLeu     151015     AspLysTyrLeuArgArgArgLeuIleLeuAsnIleSerIleValXaa     202530     LysXaaLeuSerGluLysArgXaaIleXaaMetAsnLysLysLysMet     354045     IleLeuThrSerLeuAlaSerValAlaIleLeuGlyAlaGlyPheVal     505560     AlaSerGlnProThrValValArgAlaGluGluSerProValAlaSer     65707580     GlnSerLysAlaGluLysAspTyrAspAlaAlaLysLysAspAlaLys     859095     AsnAlaLysLysAlaValGluAspAlaGlnLysAlaLeuAspAspAla     100105110     LysAlaAlaGlnLysLysTyrAspGluAspGlnLysLysThrGluGlu     115120125     LysAlaAlaLeuGluLysAlaAlaSerGluGluMetAspLysAlaVal     130135140     AlaAlaValGlnGlnAlaTyrLeuAlaTyrGlnGlnAlaThrAspLys     145150155160     AlaAlaLysAspAlaAlaAspLysMetIleAspGluAlaLysLysArg     165170175     GluGluGluAlaLysThrLysPheAsnThrValArgAlaMetValVal     180185190     ProGluProGluGlnLeuAlaGluThrLysLysLysSerGluGluAla     195200205     LysGlnLysAlaProGluLeuThrLysLysLeuGluGluAlaLysAla     210215220     LysLeuGluGluAlaGluLysLysAlaThrGluAlaLysGlnLysVal     225230235240     AspAlaGluGluValAlaProGlnAlaLysIleAlaGluLeuGluAsn     245250255     GlnValHisArgLeuGluGlnGluLeuLysGluIleAspGluSerGlu     260265270     SerGluAspTyrAlaLysGluGlyPheArgAlaProLeuGlnSerLys     275280285     LeuAspAlaLysLysAlaLysLeuSerLysLeuGluGluLeuSerAsp     290295300     LysIleAspGluLeuAspAlaGluIleAlaLysLeuGluAspGlnLeu     305310315320     LysAlaAlaGluGluAsnAsnAsnValGluAspTyrPheLysGluGly     325330335     LeuGluLysThrIleAlaAlaLysLysAlaGluLeuGluLysThrGlu     340345350     AlaAspLeuLysLysAlaValAsnGluProGluLysProAlaProAla     355360365     ProGluThrProAlaProGluAlaProAlaGluGlnProLysProAla     370375380     ProAlaProGlnProAlaProAlaProLysProGluLysProAlaGlu     385390395400     GlnProLysProGluLysThrAspAspGlnGlnAlaGluGluAspTyr     405410415     AlaArgArgSerGluGluGluTyrAsnArgLeuThrGlnGlnGlnPro     420425430     ProLysAlaGluLysProAlaProAlaProLysThrGlyTrpLysGln     435440445     GluAsnGlyMetTrpTyrPheTyrAsnThrAspGlySerMetAlaThr     450455460     GlyTrpLeuGlnAsnAsnGlySerTrpTyrTyrLeuAsnSerAsnGly     465470475480     AlaMetAlaThrGlyTrpLeuGlnTyrAsnGlySerTrpTyrTyrLeu     485490495     AsnAlaAsnGlyAlaMetAlaThrGlyTrpAlaLysValAsnGlySer     500505510     TrpTyrTyrLeuAsnAlaAsnGlyAlaMetAlaThrGlyTrpLeuGln     515520525     TyrAsnGlySerTrpTyrTyrLeuAsnAlaAsnGlyAlaMetAlaThr     530535540     GlyTrpAlaLysValAsnGlySerTrpTyrTyrLeuAsnAlaAsnGly     545550555560     AlaMetAlaThrGlyTrpLeuGlnTyrAsnGlySerTrpTyrTyrLeu     565570575     AsnAlaAsnGlyAlaMetAlaThrGlyTrpAlaLysValAsnGlySer     580585590     TrpTyrTyrLeuAsnAlaAsnGlyAlaMetAlaThrGlyTrpValLys     595600605     AspGlyAspThrTrpTyrTyrLeuGluAlaSerGlyAlaMetLysAla     610615620     SerGlnTrpPheLysValSerAspLysTrpTyrTyrValAsnGlyLeu     625630635640     GlyAlaLeuAlaValAsnThrThrValAspGlyTyrLysValAsnAla     645650655     AsnGlyGluTrpValXaaAlaAspXaaIleLysAlaCysXaaGluHis     660665670     LeuThrPheXaaPheXaaAsnLysAspLysValArgLeuAsnArgPhe     675680685     MetPheValPhePheArgTyr     690695     (2) INFORMATION FOR SEQ ID NO:24:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 21 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:24:     GluGluTyrAsnArgLeuThrGlnGlnGlnProProLysAlaGluLys     151015     ProAlaProAlaPro     20     (2) INFORMATION FOR SEQ ID NO:25:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 7 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:25:     GlnGlnGlnProProLysAla     15     (2) INFORMATION FOR SEQ ID NO:26:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 484 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:26:     MetAsnMetLysLysAlaThrIleAlaAlaThrAlaGlyIleAlaVal     151015     ThrAlaPheArgAlaProThrIleArgSerAlaSerThrValValVal     202530     GluAlaGlyAspThrLeuTrpGlyIleAlaGlnSerLysGlyThrThr     354045     ValAspAlaIleLysLysAlaAsnAsnLeuThrThrAspLysIleVal     505560     ProGlyGlnLysLeuGlnValAsnAsnGluValAlaAlaAlaGluLys     65707580     ThrGluLysSerValSerAlaThrTrpLeuAsnValArgSerGlyAla     859095     GlyValAspAsnSerIleIleThrSerIleLysGlyGlyThrLysVal     100105110     ThrValGluThrThrGluSerAsnGlyTrpHisLysIleThrTyrAsn     115120125     AspGlyLysThrGlyPheValAsnGlyLysTyrLeuThrAspLysAla     130135140     ValSerThrProValAlaProThrGlnGluValLysLysGluThrThr     145150155160     ThrGlnGlnAlaAlaProAlaAlaGluThrLysThrGluValLysGln     165170175     ThrThrGlnAlaThrThrProAlaProLysValAlaGluThrLysGlu     180185190     ThrProValValAspGlnAsnAlaThrThrHisAlaValLysSerGly     195200205     AspThrIleTrpAlaLeuSerValLysTyrGlyValSerValGlnAsp     210215220     IleMetSerTrpAsnAsnLeuSerSerSerSerIleTyrValGlyGln     225230235240     LysLeuAlaIleLysGlnThrAlaAsnThrAlaThrProLysAlaGlu     245250255     ValLysThrGluAlaProAlaAlaGluLysGlnAlaAlaProValVal     260265270     LysGluAsnThrAsnThrAsnThrAlaThrThrGluLysLysGluThr     275280285     AlaThrGlnGlnGlnThrAlaProLysAlaProThrGluAlaAlaLys     290295300     ProAlaProAlaProSerThrAsnThrAsnAlaAsnLysThrAsnThr     305310315320     AsnThrAsnThrAsnThrAsnThrAsnAsnThrAsnThrAsnThrPro     325330335     SerLysAsnThrAsnThrAsnSerAsnThrAsnThrAsnThrAsnSer     340345350     AsnThrAsnAlaAsnGlnGlySerSerAsnAsnAsnSerAsnSerSer     355360365     AlaSerAlaIleIleAlaGluAlaGlnLysHisLeuGlyLysAlaTyr     370375380     SerTrpGlyGlyAsnGlyProThrThrPheAspCysSerGlyTyrThr     385390395400     LysTyrValPheAlaLysAlaGlyIleSerLeuProArgThrSerGly     405410415     AlaGlnTyrAlaSerThrThrArgIleSerGluSerGlnAlaLysPro     420425430     GlyAspLeuValPhePheAspTyrGlySerGlyIleSerHisValGly     435440445     IleTyrValGlyAsnGlyGlnMetIleAsnAlaGlnAspAsnGlyVal     450455460     LysTyrAspAsnIleHisGlySerGlyTrpGlyLysTyrLeuValGly     465470475480     PheGlyArgVal     (2) INFORMATION FOR SEQ ID NO:27:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 21 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:27:     AlaValSerThrProValAlaProThrGlnGluValLysLysGluThr     151015     ThrThrGlnGlnAla     20     (2) INFORMATION FOR SEQ ID NO:28:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 7 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:28:     ProThrGlnGluValLysLys     15     (2) INFORMATION FOR SEQ ID NO:29:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 21 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:29:     ValLysGlnThrThrGlnAlaThrThrProAlaProLysValAlaGlu     151015     ThrLysGluThrPro     20     (2) INFORMATION FOR SEQ ID NO:30:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 7 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:30:     ThrThrProAlaProLysVal     15     (2) INFORMATION FOR SEQ ID NO:31:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 21 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:31:     LeuAlaIleLysGlnThrAlaAsnThrAlaThrProLysAlaGluVal     151015     LysThrGluAlaPro     20     (2) INFORMATION FOR SEQ ID NO:32:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 7 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:32:     AsnThrAlaThrProLysAla     15     (2) INFORMATION FOR SEQ ID NO:33:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 21 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:33:     LysLysGluThrAlaThrGlnGlnGlnThrAlaProLysAlaProThr     151015     GluAlaAlaLysPro     20     (2) INFORMATION FOR SEQ ID NO:34:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 7 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:34:     GlnGlnThrAlaProLysAla     15     (2) INFORMATION FOR SEQ ID NO:35:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 99 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:35:     MetLysValSerAlaAlaLeuLeuCysLeuLeuLeuIleAlaAlaThr     151015     PheIleProGlnGlyLeuAlaGlnProAspAlaIleAsnAlaProVal     202530     ThrCysCysTyrAsnPheThrAsnArgLysIleSerValGlnArgLeu     354045     AlaSerTyrArgArgIleThrSerSerLysCysProLysGluAlaVal     505560     IlePheLysThrIleValAlaLysGluIleCysAlaAspProLysGln     65707580     LysTrpValGlnAspSerMetAspHisLeuAspLysGlnThrGlnThr     859095     ProLysThr     (2) INFORMATION FOR SEQ ID NO:36:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 14 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:36:     SerMetAspHisLeuAspLysGlnThrGlnThrProLysThr     1510     (2) INFORMATION FOR SEQ ID NO:37:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 7 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:37:     GlnThrGlnThrProLysThr     15     (2) INFORMATION FOR SEQ ID NO:38:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 67 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:38:     LysSerThrThrCysCysTyrArgPheIleAsnLysLysIleProLys     151015     GlnArgLeuGluSerTyrArgArgThrThrSerSerHisCysProArg     202530     GluAlaValIlePheLysAspLysGluIleCysAlaAspProThrGln     354045     LysTrpValGlnAspPheMetLysHisLeuAspLysLysThrGlnThr     505560     ProLysLeu     65     (2) INFORMATION FOR SEQ ID NO:39:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 14 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:39:     PheMetLysHisLeuAspLysLysThrGlnThrProLysLeu     1510     (2) INFORMATION FOR SEQ ID NO:40:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 7 amino acids     (B) TYPE: amino acid     (C) STRANDEDNESS:     (D) TOPOLOGY: unknown     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:40:     LysThrGlnThrProLysLeu     15     __________________________________________________________________________ 

We claim:
 1. A peptide having the formula

    aXb,

wherein a is linked to X by a simple peptide bond and b is linked to X by a simple peptide bond, whereinX is a Meningitis Related Homologous Antigenic Sequence contained in the amino acid sequence of a protein or peptide found in a bacterium or virus that is an etiologic agent of meningitis, said protein or peptide is a chemokine involved in cell chemotaxis, and wherein said Meningitis Related Homologous Antigenic Sequence is at least 7 sequential amino acids and conservative substitutions or modifications thereof selected from the group consisting of:(i) the amino acid sequence of the Structural Polyprotein of a strain of Rubella virus that corresponds to AA₁₀₂ -AA₁₀₈ of said protein of the M33 strain of Rubella virus as set forth in SEQ ID NO:1; (ii) the amino acid sequence of the Structural Polyprotein of a strain of Rubella virus that corresponds to AA₈₉ -AA₉₅ of said protein of the M33 strain of Rubella virus as set forth in SEQ ID NO:1; (iii) the amino acid sequence of the Structural Polyprotein of a strain of Rubella virus that corresponds to AA₃₁₃ -AA₃₁₉ of said protein of the M33 strain of Rubella virus as set forth in SEQ ID NO:1; (iv) the amino acid sequence of the Structural Polyprotein of a strain of Rubella virus that corresponds to AA₁₀₃ -AA₁₀₉ of said protein of the Therien strain of Rubella virus as set forth in SEQ ID NO:8; (v) the amino acid sequence of the Structural Polyprotein of a strain of Rubella virus that corresponds to AA₉₀ -AA₉₆ of said protein of the Therien strain of Rubella virus as set forth in SEQ ID NO:8; (vi) the amino acid sequence of the Structural Polyprotein of a strain of Rubella virus that corresponds to AA₃₁₄ -AA₃₂₀ of said protein of the Thorion strain of Rubella virus as set forth in SEQ ID NO:8; (vii) the amine acid sequence of the Gag Polyprotein of an isolate of the HIV-1 that corresponds to AA₁₄₅ -AA₁₅₁ of the Gag Polyprotein of the LV isolate of HIV-1 as set forth in SEQ ID NO:11; (viii) the amine acid sequence of the Envelope Polypretain Precursor of an isolate of the HIV-1 that corresponds to AA₆₅₅ to AA₆₆₁ of the Envelope Polypretain Precursor of the LAV-1a isolate of HIV-1 as set forth in SEQ ID NO:14; (ix) the amine acid sequence that corresponds to AA₉₉ -AA₁₀₅ of the Lipoprotein E Precursor of Haemophilus influenzae as set forth in SEQ ID NO:17; (x) the amine acid sequence that corresponds to AA₁ to AA₅ of the Opacity-Related Protein POPM3 of Neisseria meningitidis as set forth in SEQ ID NO:20; (xi) the amine acid sequence that corresponds to A₁₂₃ to AA₁₂₉ of the Pneumococcal Surface Protein A of Streptococcus pneumoniae as set forth in SEQ ID NO:23; (xii) the amine acid sequence that corresponds to AA₁₅₁ -AA₁₅₇ of the Protein P60 Precursor of Listeria monocytogenes as set forth in SEQ ID NO:26; (xiii) the amine acid sequence that corresponds to AA₁₈₁ -AA₁₈₇ of the Protein P60 Precursor of Listeria monocytogenes as set forth in SEQ ID NO:26; (xiv) the amine acid sequence that corresponds to AA₂₄₉ -AA₂₅₅ of the Protein P60 Precursor of Listeria monocytogenes as set forth in SEQ ID NO:26; (xv) the amine acid sequence that corresponds to A₂₉₂ -AA₂₉₈ of the Protein P60 Precursor of Listeria monocytogenes as set forth in SEQ ID NO:26; (xvi) the amine acid sequence of a variant of the chemokine human Monocyte Chemoattractant Factor hMCP-1, that corresponds to AA₉₃ -AA₉₉ of hMCP-1 as set forth in SEQ ID NO:35; and (xvii) the amino acid sequence of the chemokine hMCP-3, that corresponds to AA₆₁ -AA₆₇ of hMCP-3 as set forth in SEQ ID NO: 38, whereina is an amino terminus which comprises(i) one to eight sequential amino acids from the native amino acid sequence of the protein immediately N-terminal to said X, or conservative substitutions in or modifications of said native amino acid sequence or (ii) a substituent effective to facilitate coupling of the peptide to another moiety and whereinb is a carboxy terminus which comprises(i) one to eight sequential amino acids from the native amino acid sequence of the protein immediately C-terminal to said X or conservative substitutions in or modifications of said native amino acid sequence or (ii) a substituent effective to facilitate coupling of the peptide to another moiety.
 2. An article of manufacture for use in an immunoassay capable of detecting antibodies against meningitis-causing etiologic organisms or a chemokine involved in cell chemotaxis comprising a solid support and, having bound to said support, a peptide having a formula aXb according to claim
 1. 